丘脑底核电刺激对大鼠黑质网状部及苍白球细胞外神经递质的影响  

Neurotransmitter＇ schange of extracellular fluid in substantia nigra and globus pallidus during electrical stimulation of subthalamic nucleus in rats

作　　者：初君盛[1] 张斌[1] 张建国[1] 张颖[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经外科,100050

出　　处：《中华神经外科杂志》2009年第8期742-745,共4页Chinese Journal of Neurosurgery

基　　金：北京市科技新星计划资助（2005B38）

摘　　要：目的通过电刺激正常及癫痫大鼠丘脑底核（STN），研究黑质网状部（SNr）及苍白球（GP）细胞外液中谷氨酸（Glu）、γ-氨基丁酸（GABA）的变化，探讨电刺激治疗癫痫的机制。方法正常大鼠和癫痫大鼠各20只，将刺激电极植入一侧STN，分别用130Hz和260Hz进行刺激，同时在同侧的SNr和GP收集细胞外液，用高压液相色谱法检测其Glu和GABA的含量。结果癫痫大鼠SNr的GABA基础值明显高于正常大鼠。电刺激使两组SNr的GABA明显升高。130Hz和260Hz刺激明显增高两组GP和SNr的Glu含量，但130Hz的更显著。结论SNr细胞外GABA升高在STN电刺激治疗中起重要作用。电刺激增加了GP细胞的活动，STN电刺激治疗癫痫机制不能单纯解释为“功能的毁损”。Objective To study the change of extracellular glutamate （Glu） and γ- aminobutyric acid （GABA） in substantia nigra （SNr） and globus pallidus （GP） during high frequency stimulation of subthalamic nucleus （STN） in normal and epileptic rats . Methods Two animal groups： 20 epilepsy model rats and 20 normal rats were used. Concentric bipolar electrodes were stereotaxically implanted in the unilateral STN, stimulated in high frequency 130 Hz, 260 Hz in each group. The microdialysis probes were unilaterally lowered into the ipsilateral GP and SNr. The concentrations of Glu and GABA in dialysate samples were determined by high -performance liquid chromatography （HPLC）. Results There was significant increase in the basal level GABA in SNr of epileptic rats. In both groups, electrical stimulation of STN induced increases of GABA contents in SNr. In addition, the frequency of 130 Hz provoked the maximal increase of Glu contents both in GP and SNr, whereas 260 Hz induced less effect. Conclusions The increase of GABA during electrical STN stimulation plays an important role in acting against the seizure. The GP activity is increased by STN stimulation. The impact of STN HFS should not be considered as a simple ＂functional lesion＂ of STN.

关 键 词：电刺激 丘脑底核 黑质网状部 苍白球 微透析 癫痫 

分 类 号：R686[医药卫生—骨科学]