贵州省猪繁殖与呼吸综合征病毒ORF5基因的变异分析  

Variation Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolates from Guizhou Province

作　　者：汤德元[1] 赵启祖[2] 李春燕[1] 刘霞[1] 邹兴启[2] 范运峰[2] 曾智勇[1] 朱永兴[1] 

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]中国兽医药品监察所,北京100081

出　　处：《畜牧兽医学报》2009年第8期1271-1275,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"十一五"科技支撑项目(2007BAD86B06-4);贵州省贵阳市2005年畜牧科技专项基金项目资助[(2005)筑科农字第4-11号];2007年贵州省科技厅项基金项目资助[黔科合J字(2007)2068号]

摘　　要：为了调查猪繁殖与呼吸综合征病毒ORF5基因的变异情况，笔者收集了贵州省16家规模化养殖场153份疑似PRRS的样品，通过RT-PCR检测和ORF5基因序列的测定，结果表明：所有样品之间ORF5基因的同源性为98.7%～100%，氨基酸的同源性为98.5%～100%；与美洲型代表毒株VR-2332、国内标准毒株CH-1a和欧洲型代表毒株LV基因序列的同源性分别为89.2%～90%、94.8%～95.6%、59.7%～60.2%，氨基酸序列同源性分别为88.8%～89.3%、92.7%～94.2%、54.9%～55.3%，遗传衍化关系分析表明流行毒株属于美洲型。ORF5基因发生离散性变异，可以分为3个簇，与中国其他地区分离的高致病性PRRS病毒关系密切，而与早期分离的PRRS毒株关系疏远，说明猪繁殖与呼吸综合征病毒ORF5基因存在变异现象。To investigate variation of porcine reproductive and respiratory syndrome virus （PRRSV） ORF5 gene, 153 suspected samples of PRRSV were collected from 16 large-scale farms of Guizhou province. With RT-PCR detecting and ORF5 gene sequencing, results show that among all these samples the homology of ORF5 gene was up to 98.7%-100%, and the homology of amino acids was up to 98.5%-100% ;compared with VR-2332 ,CH-1a and LV,the homologies of gene sequence were up to 89.2%-90%, 94.8%-95.6%, 59.7%-60.2% respectively, and the deduced amino acid homologies were up to 88.8%-89.3%, 92.7%-94.2%, 54.9%- 55.3% respectively. It is indicates that the epidemic strains are from the same origin and belong to Northern American genotype. The ORF5 gene from the variant can be separated into three clusters. The relationship is closed to the highly pathogenic PRRSV that separated at other parts of China,but alienate from PRRSV strain separated early. Our results indicated that the ORF5 gene of PRRSV existed variation.

关 键 词：猪繁殖与呼吸综合征 ORF5基因 变异分析 

分 类 号：S852.659.6[农业科学—基础兽医学]