鸡传染性法氏囊病病毒在DT40细胞中的增殖规律  

Proliferation of Infectious Bursal Disease Virus in the Chicken B-lymphocyte Cell Line DT40

作　　者：罗俊[1] 滕蔓[1] 樊剑鸣[1,2] 邢广旭[1] 李乔木[1] 杨艳艳[1] 张改平[1] 

机构地区：[1]河南省农业科学院河南省动物免疫学重点实验室,郑州450002 [2]郑州大学公共卫生学院毒理学教研室,郑州450001

出　　处：《畜牧兽医学报》2009年第8期1276-1280,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划(2006BAD06A04-6);河南省基础与前沿研究计划项目(082300433201)

摘　　要：为了系统地研究IBDV弱毒在DT40细胞系中的增殖规律,作者探讨了IBDV弱毒株对DT40细胞的侵染性以及不同培养条件对IBDV在该细胞中增殖的影响。结果表明,在基础维持液培养条件下,IBDV TAD和HN32个弱毒株均不需要传代适应过程即可直接感染DT40细胞,并在其中增殖;而在细胞最佳生长液条件下,IBDV可伴随宿主细胞的快速分裂而增殖,并可在较长培养时间内进行连续、大量的病毒收获。本研究表明DT40细胞可以作为深入探讨IBDV分子致病机制的良好靶细胞模型,其同时也可能为生产IBDV商品化疫苗提供一种潜在便捷的工具细胞。In the present study, the infectivity and propagation of infectious bursal disease virus （IBDV） in DT40 cells were investigated based on a systematic selection of the optimal maintenance medium for virus infection. The results showed that both of the two tested attenuated IBDV strains, TAD and HN3, could directly infect and proliferate in DT40 cells without adaptive passage. The IBDV particles could be rapidly propagated and continuously harvested at high titers for a long time, accompanying with the rapid growth of DT40 cells. Our data suggest that DT40 could be used as an ideal model for the research of the pathogenetic mechanisms of IBDV. Additionally, it could also serve as a potential system for the preparation of the commercial vaccines against IBDV.

关 键 词：IBDV DT40细胞 病毒增殖 疫苗制备 

分 类 号：S852.659.4[农业科学—基础兽医学]