氧化应激可选择性诱导细胞的NKG2D配体的表达  

Expressions of NKG2D ligands can be selectively induced by oxidative stress

作　　者：李成红[1] 崔莲仙[1] 何维[1] 马驰[1] 

机构地区：[1]中国医学科学院基础医学研究所北京协和医学院基础学院免疫学系,北京100005

出　　处：《基础医学与临床》2009年第8期789-795,共7页Basic and Clinical Medicine

基　　金：国家自然基金项目(30872362;30490244);国家高技术研究发展计划(863)项目(2006AA02Z432)

摘　　要：目的探讨氧化应激与细胞NKG2D配体表达的关系,分析氧化应激对NK细胞功能的影响。方法加H2O2诱导培养的肿瘤细胞处于氧化应激状态。用RT-PCR、Real-time PCR和流式细胞仪等方法分析细胞多种NKG2D配体的表达。用CCK-8法检测NK92细胞对肿瘤细胞的杀伤活性。结果氧化应激可诱导肿瘤细胞多种NKG2D配体的表达,不同的肿瘤细胞诱导表达的NKG2D配体不同;NKG2D配体表达上调可有效提高NK细胞的细胞毒活性,此效应可被抗NKG2D抗体所阻断。结论NKG2D配体可能在机体的免疫应答中发挥正向的调节作用。Objective To study the relationship between the expression of NKG2D ligands and oxidative stress, and to analyze the effect of oxidative stress on the function of NK cells. Methods Tumor ceils were cultured and exposed to hydrogen peroxide to develope an oxidative stress model. Then to detect the expression of NKG2D ligands in cells by Real-time PCR and Flow Cytometry. The cytotoxieity of NK ceils to tumor cells was detected and compared by CCK-8 kit before and after oxidative stress. Results The expression of NKG2D ligands was induced by oxidative stress,however the NKG2D ligands induced was variable. The up-regulation of NKG2D ligands increased the cytotoxieity of NK ceils efficiently, and this effect was blocked by anti-NKG2D antibody. Conclusion The expression of NKG2D ligands can be selectively induced by oxidative stress on tumor cells, and the improvement of the cytotoxicity of NK cells may enhance the immune responses accordingly.

关 键 词：氧化应激 NKG2D配体 NK细胞 细胞毒活性 

分 类 号：R392.1[医药卫生—免疫学]