运动对胰岛素抵抗大鼠骨骼肌脂联素受体的影响  

The effect of exercise on adiponectin receptor expression in skeletal muscle of insulin-resistant rats

作　　者：肖方喜[1] 孙晖[2] 陈璐璐[2] 尚敬[2] 舒砚文[3] 

机构地区：[1]武汉市第一医院内分泌科,武汉430022 [2]华中科技大学同济医学院附属协和医院内分泌科 [3]华中科技大学同济医学院附属协和医院心内科

出　　处：《中华物理医学与康复杂志》2009年第8期505-508,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的观察运动对高脂饮食诱导胰岛素抵抗大鼠血清脂联素（APN）及骨骼肌APN受体（AdipoR）的影响。方法将30只Wistar大鼠分为对照组及高脂组，分别给予基础饲料和高脂饲料喂养；经喂养18周后再将高脂组大鼠随机分为静息组和运动组，继续给予高脂饲料喂养，运动组同时进行游泳训练，共持续6周。于实验进行24周后测量各组大鼠体重，检测空腹胰岛素（FINS）及空腹血糖（FBG）水平，计算胰岛素敏感指数（ISI）；采用酶联免疫吸附法检测血清APN含量；选用实时荧光定量聚合酶链反应检测骨骼肌APN受体1（AdipoR1）及APN受体2（AdipoR2）mRNA的表达。结果高脂组大鼠经高脂饲料喂养18周后，其ISI较对照组明显降低，提示胰岛素抵抗模型制作成功。实验进行24周后，与对照组比较，静息组大鼠ISI显著降低，血清APN含量及骨骼肌AdipoR1、AdipoR2mRNA表达分别降低至对照组71．9％、59．9％及69．2％水平（均P〈0．05）；与静息组比较，运动组大鼠ISI明显提高（P〈0．05）；骨骼肌中AdipoR1mRNA表达亦显示提高，约是静息组的1．33倍（P〈0．01），但血清APN含量及骨骼肌AdipoR2mRNA表达组间差异均无统计学意义（P〉0．05）。结论运动干预可提高大鼠胰岛素敏感性，其机制可能与上调大鼠骨骼肌AdipoR1表达有关。Objective To observe the effects of exercise on serum adiponectin and adiponectin receptor （AdipoR） level in skeletal muscle of insulin-resistant rats. Methods A total of 30 healthy male rats were randomly divided into a control group （NC, n =8） and a high-fat group （HF, n =22） , fed with normal chow and high fat di- et, respectively. Eighteen weeks later, the high-fat group was randomly divided into a high-fat diet control group （HC, n = 10） and an exercise group （HE, n = 12）. The HC and HE group were continually fed with high fat diet, while the HE group was administered with swimming training for 6 weeks in addition at the same time. After 24 weeks, the insulin sensitivity index was calculated,and serum adiponectin level was detected by using ELISA. The expressions of AdipoR mRNA in skeletal muscle were detected with real-time fluorescence quantitative polymerase chain reaction （PCR）. Results After 18 weeks, compared to NC group, the insulin sensitivity index of HF group decreased significantly. It suggested that insulin resistance appeared in HF group. Twenty-four weeks later, compared to NC group, the ISI of HC group was significantly decreased, meanwhile the level of serum adiponectin, expression of AdipoR1 and AdipoR2 mRNA in skeletal muscle of HC group were 71.9% , 59.9% and 69.2% of those of the NC group, respectively; cor0pared to HC group, the ISI was increased significantly by exercise, meanwhile the expression of AdipoR1 mRNA in skeletal muscle was significantly increased by 1.33 times, however the level of serum adiponectin and the expression of AdipoR2 mRNA in skeletal muscle were not altered in HE group. Conclusion Six weeks of exercise improves insulin sensitivity through increasing the expression of AdipoR1 mRNA in skeletal muscle.

关 键 词：运动 胰岛素抵抗 脂联素 脂联素受体 

分 类 号：R87[医药卫生—运动医学]