磁疗对大鼠创伤性脑组织损伤局部淋巴细胞浸润及神经细胞凋亡的影响  

The effects of magnetotherapy on local lymphocyte infiltration and neurocytes apoptosis after traumatic brain injury of rats

作　　者：王玉苹[1] 宋秀豹[1] 洪其生[1] 李卫[2] 

机构地区：[1]暨南大学附属第一医院康复理疗科,广州510630 [2]暨南大学附属第一医院神经外科,广州510630

出　　处：《中华物理医学与康复杂志》2009年第8期524-527,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：广东省自然科学基金（8451063201000363）;广东省医学科研基金（A2008353）;广东省中医药局建设中医药强省科研课题基金（2008101）

摘　　要：目的研究磁疗对大鼠创伤性脑组织损伤后神经功能的保护作用及其可能机制。方法制作20只大鼠创伤性脑组织损伤模型，随机分为磁疗组和非磁疗组。磁疗组头部创面区接受磁疗，磁场强度为表面强度0．15T．旋转时强度0．1T，转速为3000转／min，每天治疗2次，每次20min，共10d。非磁疗组不接受磁疗。伤后第11天测定大鼠左后肢腓肠肌的收缩力，随后取脑组织标本，免疫组化方法观察损伤灶周边分化抗原簇4阳性（CD4＋）、分化抗原簇8阳性（CD8＋）细胞浸润及细胞凋亡情况，分析各指标间的关系。结果伤后第11天，磁疗组大鼠损伤灶周围的CD4＋、CD8＋细胞浸润及细胞凋亡数量明显少于非磁疗组，而磁疗组左后肢腓肠肌的收缩力却明显强于非磁疗组。结论磁疗可能通过抑制脑组织损伤灶局部的淋巴细胞浸润及神经细胞凋亡，减轻脑组织的进一步损伤，保护了神经功能。Objective To study the neuro-protective effects of magnetotherapy after traumatic brain injury of rats. Methods Traumatic brain injury models were created in 20 rats and divided randomly into magnetotherapy and non-magnetotherapy groups. The rats in magnetotherapy group received magnetotherapy on the cranial surface of traumatized region twice daily, each for 20 min, over 10 d. The intensity of magnetic field was 0.15 T at the surface, and 0.1 T during spinning at 3000 rpm. The contraction strength of gastrocnemius of each rat＇s left hind limb was measured on the 1 lth d after trauma. The rats were then sacrificed and brain tissue samples were taken. The infiltrations of CD4 ＋ and CD8 ＋ cells and apoptosis in the area around the injury lesion were observed with immunohisto- chemical methods. The relationship among the indexes was analyzed. Results At the 1 lth d after brain injury, the quantities of CD4 ＋ and CD8 ＋ cells and apoptotic ceils in magnetotherapy group were all obviously less than those in non-magnetotherapy group. Average contraction strength of gastrocnemius in magnetotherapy group was significantly stronger than that in non-magnetotherapy group. Conclusions Magnetotherapy can relieve the secondary effects of brain injury and induce neuro-protection effects. This may involve decreasing the local infiltrations of CD4 ＋ and CD8 ＋ and apoptotic ceils in the area around the injury lesion.

关 键 词：脑损伤 磁疗 凋亡 

分 类 号：R454.1[医药卫生—治疗学] R651.15[医药卫生—临床医学]