羟乙基淀粉130/0.4改善大鼠心肌缺血再灌注损伤  

Protective effects of hydroxyethyl starch 130/0.4 against myocardial ischemia-reperfusion injury in rats

作　　者：孙海静[1] 刘波[2] 邹最[1] 王亚华[1] 朱秋峰[1] 袁红斌[1] 石学银[1] 

机构地区：[1]第二军医大学长征医院麻醉科,上海200003 [2]沈阳军区总医院麻醉科,沈阳110016

出　　处：《第二军医大学学报》2009年第8期947-949,共3页Academic Journal of Second Military Medical University

基　　金：军队"十一五"科技攻关项目(08G071)~~

摘　　要：目的:观察羟乙基淀粉(HES)130/0.4(万汶)对缺血再灌注(I/R)损伤心肌的干预作用,探讨其可能的作用机制。方法:24只SD大鼠随机均分为4组(n=6):假手术(S)组、缺血再灌注(IR)组、白蛋白-缺血再灌注(A-IR)组、HES-缺血再灌注(H-IR)组。后3组建立在体大鼠心肌I/R模型,分别在缺血25min时股静脉持续泵入生理盐水、5%白蛋白或HES130/0.4,假手术组行开胸手术但不结扎左冠状动脉前降支。再灌注180min处死大鼠,取心肌组织观察病理学改变;处死前颈动脉采血ELISA法测定TNF-α、IL-1β浓度;测定心肌组织NF-κB活性。结果:H-IR组心肌组织损伤病理学改变较IR组、A-IR组减轻。IR组、A-IR组、H-IR组血清TNF-α、IL-1β水平和心肌NF-κB活性明显高于S组(P<0.05),但其中H-IR组血清TNF-α、IL-1β水平及心肌NF-κB活性上升程度不及IR组、A-IR组(P<0.05)。结论:羟乙基淀粉130/0.4能减轻缺血再灌注所致心肌病理损伤,可能与其抑制NF-κB的活性、减少促炎细胞因子的释放有关。Objective：To explore the influence of hydroxyethyl starch（HES） 130/0.4 on myocardial ischemia-reperfusion （I/R） injury in rats and the possible mechanism.Methods： Twenty-four SD rats were evenly randomized into four groups（n=6）：the sham operation group,the I/R（IR） group,albumin ＋ I/R（A-IR） group,and HES＋I/R（H-IR） group; rats in the latter three groups were made into I/R models and were treated respectively with 7.5 ml/kg saline,5% albumin and HES 130/0.4 through femoral vein at 25 min of ischemia.At 180 min of reperfusion,animals were sacrificed and the pathological changes of myocardium were observed.Serum concentrations of TNF-α and IL-1β and the myocardial NF-κB activity were also measured.Results： Histological examination showed that the injury in H-IR group was ameliorated compared with those in IR and A-IR groups.NF-κB activity and TNF-α ,IL-1β concentrations in the sham operation group were significantly lower than those of the other 3 groups （P〈0.05）; and the increases of the above parameters in H-IR group were smaller than those of the IR and A-IR groups （P〈0.05）.Conclusion： HES 130/0.4 can improve myocardial function and attenuate ischemia-reperfusion injury,and the mechanism might be related to the inhibition of myocardial NF-κB activity and reduction of proinflammatory factors.

关 键 词：心肌再灌注损伤 肿瘤坏死因子Α 白细胞介素1Β NF-κB 羟乙基淀粉 

分 类 号：R542.2[医药卫生—心血管疾病]