mRNA差别显示技术研究进展及其在水稻中的应用  

Progress and Application of mRNA Differential Display in Rice

作　　者：唐江云[1] 张涛[2,3] 郑家奎[1,2,3] 

机构地区：[1]重庆大学生物工程学院,重庆400044 [2]四川省农业科学院水稻高粱研究所,泸州646100 [3]国家水稻改良中心四川泸州分中心,泸州646100

出　　处：《生物技术通报》2009年第8期56-59,共4页Biotechnology Bulletin

基　　金：四川省"十一五"攻关项目;四川省农科院青年基金项目

摘　　要：随着基因组计划的顺利实施,大量的生物信息被解析,分离和鉴定差异表达基因已成为分子生物学研究的热点。mRNA差别显示技术(DDRT-PCR)是目前有效筛选、分离差异表达基因的方法之一。就DDRT-PCR的基本原理、存在的问题及相应的改进方法作了简要概述。阐明了该技术在水稻生长发育、杂种优势、抗逆性基因研究中的应用、取得的成绩,最后对该技术在水稻突变体及抗药性上的应用前景做了有益探讨。With the development of Genome Project and analysis of massive biological information, separation and identification of difference expressing gene have become a hot issue in molecular biology research. The mRNA differential display （DDRT-PCR） is one of effective methods for screening and isolating differentially expressed genes. The principles and problems and improvements of DDRT- PCR technique were introduced. The usability and achieved research results by using this method were summarized from the aspects of rice development gene, heterosis gene and the resistance gene. The prospective application of DDRT-PCR in paddy rice mutant and drug-resistance to agricultural chemicals research was also explored in the paper.

关 键 词：MRNA差异显示技术 水稻 杂种优势 抗性 

分 类 号：S511[农业科学—作物学]