石斛兰dfr基因植物表达载体的构建  

Plant Expression Vectors Construction of dfr Gene from Dendrobium

作　　者：潘丽晶[1] 范干群[1] 张妙彬[1] 肖杨[1] 曹友培[1] 

机构地区：[1]珠海市农业科学研究中心,珠海519070

出　　处：《生物技术通报》2009年第8期71-75,共5页Biotechnology Bulletin

基　　金：广东省科技计划项目(2006B20130004)

摘　　要：石斛兰花瓣缺乏橙色、蓝色,这与其二氢黄酮醇4-还原酶(dihydroflavonol 4-reductase,DFR)活性有着密切的关系。根据已报道的石斛兰dfr基因序列设计引物,从Den.Burana Emerald中分离了dfr基因。将该基因序列正向与反向连接到植物表达载体pCAM BIA1301中,并由组成型启动子CaMV35S驱动,成功构建了dfr基因的正义和反义植物表达载体pC-SDN1和pCSDN2,并导入根癌农杆菌(Agrobacterium tumefacien)EHA105,以期利用转基因技术培育出石斛兰花色新品种。The Dendrobium petals are devoid of some colors such as orange and blue, which is greatly due to the activity of dihydroflavonol 4-reductase （ DFR）. A dfr gene was amplified from Den. Burana Emerald flower with the specific primers according to the reported dfr gene from other Dendrobium species. The sense and antisense chains of dfr gene under the constitutive promoter CaMV35S were inserted into the plant expression vector pCAMBIA1301 to construct sense and antisense recombinant vectors pC-SDN1 and pC-SDN2, both of which were transformed into Agrobacterium tumefacien EHA105 respectively. Thus,it would help to cultivate novel color species of Dendrobium.

关 键 词：石斛兰 dfr基因 正义 反义 植物表达载体 

分 类 号：S682.31[农业科学—观赏园艺]