复方丹参滴丸HPLC数字化指纹图谱研究  

Digitized HPLC fingerprints of Compound Danshen Dropping Pills

作　　者：孙国祥[1] 宋宇睛[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110015

出　　处：《中成药》2009年第8期1152-1155,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金重大研究计划课题(90612002)

摘　　要：目的:建立复方丹参滴丸HPLC数字化指纹图谱。方法:采用反相高效液相色谱法,以色谱指纹图谱相对指数Fr为指标优化确定试验条件,用"中药色谱指纹图谱超信息特征数字化评价系统3.0"评价建立其数字化指纹图谱。结果:测定了10批复方丹参滴丸HPLC指纹图谱,以原儿茶醛为参照物峰,确定15个共有指纹峰,获得了判别其质量的重要数字化信息,并以双定性双定量相似度法评价其批间质量稳定性。结论:所建立的数字化指纹图谱能清晰反映复方丹参滴丸真实质量。AIM： To establish a digitized HPLC fingerprint of Compound Danshen Dropping PilLs （CDDP）. METHODS： The RP-HPLC condition was optimized by the chromatographic fingerprint relative index （Fr）. The chromatographic fingerprints were characteristically digitized and evaluated by the software of the digitized evaluation system of Traditional Chinese Medicine （TCM） with the super information characteristics. RESULTS： 15 copossessing peaks were selected as the fingerprint peaks of CDDP by taking protocatechualdehyde peak as the referential peak to establish the digitized fingerprint and the important digitized information for its quality control was obtained. The stabilities among different lots of samples were evaluated by the dual qualitative and dual quantitative similarity method. CONCLUSION： The fingerprints can perfectly reflect the authentical quality of CDDP.

关 键 词：复方丹参滴丸 HPLC 数字化指纹图谱 色谱指纹图谱相对指数Fr 双定性双定量相似度法 

分 类 号：R927.1[医药卫生—药学]