BTLA介导的负调共刺激信号对小鼠心脏移植受体Treg的影响  

Influence of Treg of recipients by HVEM gene transfection on mice cervical heterotopic heart transplantation

作　　者：冯嘉瑜[1] 肖亚[1] 范明齐[1] 黄赤兵[1] 张艮甫[1] 王平贤[1] 贾维胜[1] 方针强[1] 

机构地区：[1]第三军医大学新桥医院泌尿外科,重庆400037

出　　处：《重庆医学》2009年第17期2142-2143,2145,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(30571863);重庆市自然科学基金资助项目(CSTC;2007BB5015);第三军医大学校管课题资助项目(2004D109)

摘　　要：目的探讨人疱疹病毒侵入介导因子(HVEM)基因突变体腺病毒载体对小鼠心脏移植受体Treg(调节性T细胞)的影响。方法HVEM基因突变体腺病毒载体转染心脏移植物,行颈部异位心脏移植后观察其对受体小鼠Treg数量和功能的影响,并检测该腺病毒对供受体混合淋巴细胞培养体系中IL6和TGF-β的影响。结果HVEM突变体腺病毒载体转染心脏移植物后对移植受体Treg数量有增加,功能有增强的作用,移植物存活时间较对照组显著延长;该腺病毒处理供受体混合淋巴细胞培养体系后,培养上清液中IL-6的表达较对照组显著上调。结论HVEM突变体腺病毒载体在延长小鼠心脏移植物存活同时提高受体Treg的数量,并显著上调了其相关细胞因子的表达,提示HVEM-BTLA通路通过干预受体Treg功能是延长移植物存活的可能机制。Objective To observe the influence of Treg of recipients by HVEM gene transfection on mice cervical heterotopic heart transplantation. Methods Adenovirus expression vector Adv-mHVEM-IRES2-EGFP was constructed with mHVEM and EGFP by means of ADEasy system. Transferred the heart allografts by the vector before cervical heterotopic heart transplantation. The adenovirus vector was used in mixed lymphocyte reaction（MLR） and detected expression of IL6. Results Quantity of Treg in heart transplantation recipients was up-regulation markedly. Expression of IL6 in MLR was up-regulation markedly on 12h, 24h, 48h,72h. Conclusion The recombinate vector could prolong heart allografts survival time by up-regulation recipients＇ quantity of Treg.

关 键 词：人疱疹病毒侵入介导因子 B T淋巴细胞弱化因子 调节性T细胞 心脏移植 

分 类 号：R654.2[医药卫生—外科学]