显齿蛇葡萄叶提取物对大鼠肝癌前病变模型抗氧化酶活性的影响  

Effects of Ampelopsis Grmsedntara Fxtract on the Serum and Liver Antioxidative Activities in Initial Stage of Rat Chemical Hepato-carcinogenesis

作　　者：钟正贤[1] 陈学芬[1] 李燕婧[1] 杨秀芬[2] 

机构地区：[1]广西中医药研究院,广西南宁530022 [2]广西中医学院药学院,广西南宁530002

出　　处：《中医药导报》2009年第8期1-3,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(30660228)

摘　　要：目的:探讨显齿蛇葡萄叶提取物在大鼠肝脏化学致癌初期对血清及肝脏抗氧化酶活性的影响,从而进一步探索其抗致癌作用的可能机制。方法:按改良的Solt Farber方法建立大鼠肝癌前病变模型,用显齿蛇葡萄叶水提物及总黄酮饲养大鼠6周后,取血及肝脏测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)、谷胱甘肽-S-转移酶(GSH-ST)、过氧化氢酶(CAT)、丙二醛(MDA)及r-谷氨酰转肽酶(r-GT)。结果:显齿蛇葡萄叶水提物及总黄酮在大鼠肝脏化学致癌初期对血清和肝脏中SOD、GSH-px、GST、CAT的活性有明显回升作用,降低MDA含量,并能抑制癌前病变标志物r-GT活性表达。结论:显齿蛇葡萄叶提取物具有抗氧化和抑制癌前病变标志物的作用,可能是显齿蛇葡萄叶的抗致癌机制之一。Objective：To study effect from extract of Ampelopsis grmsedntara （AGE）on serum and liver antioxidative activities and r-glutamine trans- peptidase （r-GT） activity in initial stage of rat chemical hepato-carcinogenesis and invesdgate possible mechanism of its anti-carcinogenesis. Methods：Hepatic preneoplastic lesions in rats were induced by modified SOh-farber protocol. The activities of serum superoxide dismutase（SOD）,glutathione peroxidase（GSH-px）,glutathione-S-transferase（GST）, catalase （CAT）, r-glutamine transpeptidase （r-GT） and the content of malondialdehyde（MDA）were measured with colorimetrie method in the rats administered with water extract and total SOD,GSH-px, GST,CAT were increased, decreasing the content flavone for 6 weeks. Results：The activities of serum and liver of MDA and r-GT activity of initial stage of rat chemical hepato- carcinogenesis marker were inhibited after administrated AGE. Conclusion：AGE have antioxdative activities and inhibited in initial stage marker of rat chemical hepato- carcinogenesis effects,and it may be one of the anti-carcinogenic mechanisms of AGE.

关 键 词：显齿蛇葡萄叶 化学致癌 抗氧化酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]