HPLC法测定清血糖浆中绿原酸的含量  

Determination of Caffeotannic Acid in Qingxue Syrup With HPLC Method

作　　者：任贻军[1] 周菁丽[1] 李纪元[1] 胡刚[1] 

机构地区：[1]华中科技大学同济医学院附属荆州医院,湖北荆州434020

出　　处：《中医药导报》2009年第8期71-72,共2页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立清血糖浆中绿原酸的高效液相含量测定方法。方法:色谱柱为Eclipse XDB-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈:0.4%磷酸溶液(11∶89),流速为1 mL/min,检测波长为327 nm。结果:绿原酸在2.44-39.00μg/mL范围内呈良好的线性关系r,=0.9998,平均回收率为98.71%,RSD%=1.9%。结论:该方法简便、灵敏、准确、重复性好,可用于清血糖浆的质量控制。Objective： To establish HPLC method for determination of caffeotannic acid in Qingxue syrup. Methods： The chromatographic column was Eclipse XDB-CIs column（250 mm×4.6 mm, 5μm） with mobile phase of acetonitrile： 0.4% orthophosphoric acid（l 1：89）, flow rate was 1 mL/min, detection wavelength was 327 nm. Results： Caffeotannic acid showed a linear relationship at the range of 2.44-39.00 μg/mL. Conclusion： The method is simple, sensitive, accurate with good reproducibility, can be used as quality control of Qingxue syrup.

关 键 词：绿原酸 高效液相色谱法 清血糖浆 

分 类 号：R927.2[医药卫生—药学]