环氧化酶-2在肺纤维化大鼠中的表达及其意义  

Signification and expression of cyclooxygenase-2 in rats with bleomycin-induced pulmonary fibrosis

作　　者：杨俊玲[1] 孙中美[1] 林庆艳[1] 

机构地区：[1]吉林大学第二医院呼吸内科,长春130041

出　　处：《中国免疫学杂志》2009年第8期745-747,751,共4页Chinese Journal of Immunology

基　　金：吉林省科技厅资助课题(200505133)

摘　　要：目的:观察环氧化酶-2(COX-2)在肺组织中的分布及其抑制剂对博莱霉素(BLM)诱导的大鼠肺纤维化模型的干预作用。方法:雄性SD大鼠72只随机分为3组,分别为对照组、模型组、塞来昔布组。采用免疫组织化学方法检测各组急性肺泡炎期肺部COX-2的表达和分布;HE和Masson染色方法观察肺泡炎和肺纤维化的程度。结果:(1)在肺纤维化早期,模型组和塞来昔布组均可见COX-2的表达,其主要分布在细支气管上皮细胞中,以模型组表达显著。(2)与对照组相比,模型组和塞来昔布组肺泡炎改变明显(P<0.01);塞来昔布干预组的肺泡炎症与模型组比较有所减轻(P<0.05)。(3)于实验第28、42天,模型组和塞来昔布组均可见肺纤维化改变,与对照组比较差异显著(P<0.01);但塞来昔布干预组与模型组比较无显著性差异(P>0.05)。结论:COX-2在肺纤维化早期高度表达,其主要分布在细支气管上皮细胞中;COX-2抑制剂可减轻早期肺泡炎症,对后期肺纤维化无明显抑制作用。Objective：To investigate the distribution and expression of the cyclooxygenase-2 （COX-2） in lung tissues and the effect of its inhibition in bteomycin （BLM）-induced pulmonary fibrosis in rats.Methods：72 male SD rats were randomly divided into three groups：the control group, model group and celecoxib group. Immunohistochemical method was used to detect the distribution of COX-2 in the stage of acute pulmonary alveolitis. HE and Masson staining methods were used to evaculate the degree of alveolitis and pulmonary fibrosis. Results： （ 1 ） In early stage of pulmonary fibrosis except in the control group, COX-2 expression was found in small bronchial epithelial cells in the other two groups, especially the model group. （2） Compared with the control group, the other two groups had alveolitis（ P 〈 0.01 ）. Alveolitis in the celecoxib group was hghter than in the BLM group （ P 〈 0. 05）. （3） On 28th and 42th day, compared with the control group, the other two groups had polmonary fibrosis. Conclusion： COX-2 had a higher expression in small bronchial epithelial cells in lungs of bleomycin （BLM）-induced pulmonary fibrosis. Cyclooxygenase-2 inhibitor could reduce pulmonary inflammation in early stage, but couldn＇ t inhibit pulmonary fibrosis in the late stage.

关 键 词：环氧化酶-2 肺纤维化 环氧化酶-2抑制剂 

分 类 号：R563.9[医药卫生—呼吸系统]