菊花幼嫩花瓣愈伤组织的诱导和植株再生  

Callus Induction and Plant Regeneration of Young Chrysanthemum Petal

作　　者：司怀军[1] 戴朝曦[1] 于品华[1] 王蒂[1] 王清[1] 

机构地区：[1]甘肃农业大学农业生物工程研究所

出　　处：《甘肃农业大学学报》1998年第2期175-177,174,共4页Journal of Gansu Agricultural University

摘　　要：以菊花幼嫩花瓣作为供体材料，ＭＳ作为基本培养基，在激素组合为６ＢＡ１～３ｍｇ／Ｌ＋ＮＡＡ１ｍｇ／Ｌ或２，４Ｄ１～３ｍｇ／Ｌ＋ＫＴ１ｍｇ／Ｌ的范围内均能诱导出愈伤组织，但以ＭＳ附加６ＢＡ２ｍｇ／Ｌ和ＮＡＡ１ｍｇ／Ｌ的诱导率最高，可达１００％。将愈伤组织转移到ＭＳ＋６ＢＡ２ｍｇ／Ｌ＋ＮＡＡ０２ｍｇ／Ｌ的培养基上，分化芽的频率为７７８％。将３ｃｍ左右的茎段转移到不含激素的ＭＳ培养基或ＭＳ＋ＮＡＡ０１ｍｇ／Ｌ的培养基上，生根率可达９５％以上。Young petal of chrysanthemum was used as the donor tissue. callus was induced in MS media with hormone combination 6BA 1～3 mg/L + NAA 1 mg/L or 2,4 D 1～3 mg/L+KT 1 mg/L.The highest callus induction frequency (100 %) was obtained in the medium of MS supplemented with 6BA 2 mg/L and NAA 1 mg/L. After the callus was transferred to the regeneration medium (MS+6BA 2 mg/L+NAA 0 2 mg/L), the shoot regeneration frequency reached 77 8 %. In order to induce roots, the 3 cm shoots were transferred to the root induction media (MS+NAA 0 1 mg/L or hormone free), more than 95 % root induction frequency was obtained.

关 键 词：菊花 花瓣 愈伤组织 植株再生 

分 类 号：S682.110.3[农业科学—观赏园艺]