葛根素及其纳米粒在Caco-2细胞模型的吸收机制研究  

Study on Absorption Mechanism of Puerarin and Its Nanoparticles across Caco-2 Cell Model

作　　者：刘西京[1,2] 贾强[1,2] 汪程远[3] 王乃利[1,2] 

机构地区：[1]深圳清华大学研究院,广东深圳518057 [2]深圳中药及天然药物研究中心,广东深圳518057 [3]重庆工商大学药物化学与化学生物学研究中心,重庆400067

出　　处：《中药材》2009年第8期1252-1255,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:研究葛根素及其纳米粒在Caco-2细胞模型中的吸收机制。方法:用Caco-2细胞单层模型来考察时间、药物浓度对葛根素及其纳米粒吸收的影响。采用高效液相色谱法检测葛根素浓度,计算其表观通透系数(Papp)。结果:葛根素的PLGA纳米粒能显著改善药物的吸收;药物的吸收不是简单的被动扩散;药物对Caco-2细胞单层完整性无影响,并非通过胞旁作用吸收。结论:葛根素及其纳米粒在Caco-2细胞模型中的吸收主要是被动转运,纳米制剂可提高药物在小肠的吸收。Objective ：To study the absorption mechanism of puerarin and its nanopartieles across Caco-2 cell model. Methods ：The Caco-2 monolayer model was used to explore the effect of time and drug concentration on the absorption of puerarin and its nanoparticeles. The drug concentration was determined by high-performance liquid chromatography and the Papp was caculated. Results：The puerarin-PLGA-NP could significantly improve the absorption of puerarin. Puerarin was not only absorbed simply through passive diffusion, but also had relations with high dispersion of nanoparticles, increase of affinity and interaction with intestinal mucous membrane. Conclusion：The main mechanism of puerarin and its nanoparticles across Caco-2 monolayer model is passive transference. Puerarin-NP could increase the absorption of the drug in intestinal epithlial.

关 键 词：葛根素 纳米粒 CACO-2细胞模型 吸收 

分 类 号：R285.5[医药卫生—中药学]