黄芩苷、小檗碱及黄芪多糖对醛糖还原酶的体外作用及合并效应研究  

Study on Effects of Baicalin, Berberine and Astragalus Polysaccharides and Their Combinative Effects on Aldose Reductasein vitro

作　　者：杨红舟[1] 周明眉[1] 赵爱华[2] 邢淑宁[1] 范自全[1] 贾伟[1] 

机构地区：[1]上海中医药大学/中医方证与系统生物学中心,上海201203 [2]上海交通大学药学院,上海200240

出　　处：《中药材》2009年第8期1259-1261,共3页Journal of Chinese Medicinal Materials

基　　金：上海市科委中药现代化招标项目(06DZ19713);上海市科委创新团队项目(06DZ05906)

摘　　要：目的:建立醛糖还原酶(A ldose Reductase,AR)抑制剂的体外筛选模型,考察黄芩苷、小檗碱及黄芪多糖对AR的体外抑制作用及三者配伍的合并效应。方法:测定不同质量浓度的黄芩苷、小檗碱、黄芪多糖以及阳性对照药(依帕司他)对AR的抑制率;结合正交t值法评价3种药物配伍后对AR作用的合并效应。结果:300μg/mL的黄芩苷和小檗碱对AR的抑制率分别为(88.4±7.4)%、(69.0±9.4)%,二者合用时对AR的抑制作用表现为拮抗;黄芪多糖则无AR抑制活性。结论:黄芩苷、小檗碱体外能显著抑制AR的活性,是良好的醛糖还原酶抑制剂。Objective ： To observe the activities of baicalin, berberine and Astragalus polysaccharides and their combinative effects on aldose reductase （AR） by a screening model of aldose reductase inhibitor （ARI） in vitro. Methods ： The inhibition of AR by baicalin, berberine and Astragalus polysaccharides and positive drug （Epalrestat） in different concentrations were evaluated, and their combinative effects were studied according to orthogonal t design. Results ： Baicalin and berberine had remarkable inhibitory effects on AR, the inhibitory rates were （88.4±7.4）% and （69.0 ±9.4）% at the coneentration of 300 μg/mL. However, the combinative effect of the inhibition on AR by the two compounds was antagonistic action. Astragalus polysaccharides had no activity of inhibition on APt. Conclusion： Baicalin and berberine are the potential AR inhibitors as they can inhibit the activity of AR in vitro.

关 键 词：醛糖还原酶 黄芩苷 小檗碱 黄芪多糖 合并效应 正交T值法 

分 类 号：R285.5[医药卫生—中药学]