新城疫病毒F_(48)E_8株核衣壳蛋白基因的克隆及其酶切分析  

Cloning and Restriction Endonuclease Analysis of the Nucleocapsid Protein Gene of NDV Strain F 48 E 8

作　　者：姜焱[1] 吴艳涛[1] 刘伟忠[1] 刘秀梵[1] 张如宽[1] 

机构地区：[1]扬州大学农学院动物医学系

出　　处：《中国兽医学报》1998年第4期325-327,共3页Chinese Journal of Veterinary Science

基　　金：江苏省自然科学基金;江苏省教委自然科学基金

摘　　要：根据已发表的新城疫病毒（ＮＤＶ）核衣壳蛋白（ＮＰ）基因序列，设计合成１对长度为３２ｍｅｒ的引物。ＲＴ－ＰＣＲ扩增ＮＤＶＦ４８Ｅ８株、Ｕｌｓｔｅｒ株、ＬａＳｏｔａ株的ＮＰ基因，产物经琼脂糖凝胶电泳分析，均呈现１条长１．５ｋｂ左右的特异性带。将Ｆ４８Ｅ８株扩增产物克隆入ｐＵＣ１８载体，经限制性内切酶分析证实为ＮＰ基因。According to the reported NP gene sequence of NDV, a pair of 32 mer primers was designed and synthesized. Then the NP genes of three NDV strains (F 48 E 8, Ulster, La Sota) were amplified by reverse transcription polymerase chain reaction (RT PCR). The amplified products were analyzed by agarose gel electrophoresis, all appeared a specific fragment about 1.5 kb in length as expected. The RT PCR product of F 48 E 8 strain was cloned into the pUC18, then tested by restriction endonuclease analysis, the result suggested it was the NP gene of NDV F 48 E 8 strain.

关 键 词：新城疫病毒 核衣壳蛋白基因 RT-PCR技术 

分 类 号：Q78[生物学—分子生物学] S852.65[农业科学—基础兽医学]