应用单克隆抗体夹心酶联免疫吸附试验检测兔出血症病毒  

Detection of Rabbit Hemorrhagic Disease Virus by Enzyme linked Immunosorbent Assay

作　　者：孙智锋[1] 杜念兴[2] 徐为燕[2] 

机构地区：[1]上海市农业科学院畜牧兽医研究所 [2]南京农业大学兽医微生物研究室

出　　处：《中国兽医学报》1998年第4期334-337,共4页Chinese Journal of Veterinary Science

基　　金：农业部八五重点课题

摘　　要：应用建立的单克隆抗体夹心酶联兔疫吸附试验（ＭｃＡｂ－ＥＬＩＳＡ），检测了人工感染兔出血症病毒（ＲＨＤＶ）ＤＪＲＫ细胞毒、肝毒的兔以及自然感染ＲＨＤＶ的兔的组织样品。结果表明，感染死亡兔的肝、脾、肾、骨髓样品病毒抗原的检出率为１００％，淋巴结和肌肉的检出率分别为９７．５％和７９．５％。ＭｃＡｂ－ＥＬＩＳＡ能检出肌肉中血凝试验不能检出的ＲＨＤＶ抗原。此外，还用ＭｃＡｂ－ＥＬＩＳＡ检测了肝毒人工感染兔血中ＲＨＤＶ的动态，并对１０份兔出血症脏器灭活苗的效价作了滴定。Rabbit tissue samples collected from different sources were examined simultaneously by a double sandwich ELISA (McAb ELISA) and hemagglutination (HA) test. RHDV antigen was found by McAb ELISA from Liver, Spleen, Kidney and bone marrow from all rabbits infected naturally or inoculated with infected DJRK cell culture. The positive rates of popliteal lymphaden and muscle were 97.5% and 79.5%, respectively. The McAb ELISA could be used for the detection of RHDV antigen in most muscle samples which could not be detected by HA. Furthermore, the dynamic changes of RHDV in blood of the artificially infected rabbits were observed and the effective value of RHD vaccine, inactived was titered by the McAb ELISA.

关 键 词：兔出血症病毒 检测 McAb-ELISA方法 

分 类 号：S854.43[农业科学—临床兽医学] S852.65[农业科学—兽医学]