一株反硝化聚磷菌的筛选鉴定及特性研究  

Screening and identification of denitrifying dolyphosphate-acculating organisms and character

作　　者：苏俊峰[1] 黃廷林 叶羡婧[1] 李倩[1] 刘燕[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《西安建筑科技大学学报（自然科学版）》2009年第4期580-582,586,共4页Journal of Xi'an University of Architecture & Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(50778147);陕西省自然科学基础研究基金项目(SJ08-ZT07);陕西省教育厅专项科研计划项目(09JK540)

摘　　要：采用SBR反应器,通过间歇曝气方式对底泥体系中反硝化聚磷菌进行了筛选和富集.从底泥中分离出1株反硝化聚磷细菌SYJ1,经过生理生化鉴定和16SrDNA测序,建立了系统发育树,鉴定出细菌SYJ1属于Acinetobacter sp.菌株SYJ1在好氧条件下培养48 h后,TP最终去除率为68.63%,在厌氧条件下培养48h后,NO2--N及NO-3-N的去除率分别为94.56%和100%.By adopting sequencing batch reactor, selection and enrichment of denitrifying polyphosphate-accumulating organisms in the sediment using intermit tent aeration mode. Denitrifying polyphosphate-accumulating organism SYJ1 isolated from the sediment. With the aid of sequencing the 16SrDNA established the phylogenic tree, denitrifying polyphosphate-aceumulating organism SYJ1 was identified in physiologically and bioehemically. SYJ1 was Aeinetobacter sp . The removal rates of TP by strain SYJ1 was 68.63%, and anaerobic culture the removal rates of NO2-N and NOa N by strain SYJ1 was 94.56% and 100% after aerobic culture 48 hours.

关 键 词：反硝化聚磷菌 筛选 16SrDNA 

分 类 号：X703[环境科学与工程—环境工程]