线粒体DNA 5178A/C多态性与2型糖尿病患者视网膜病变的相关性研究  

Association of the Mitochondrial DNA 5178A/C Polymorphism with Diabetic Retinopathy in Chinese Type2 Diabetic Patients

作　　者：宋晓艳[1,2] 鹿斌[1] 董雪红[1] 杨叶虹[1] 刘瑜[1] 陈凤玲[1] 胡仁明[1] 

机构地区：[1]复旦大学附属华山医院内分泌代谢科,复旦大学内分泌糖尿病研究所,上海200040 [2]解放军总医院第二附属医院内分泌科,北京100091

出　　处：《中国临床医学》2009年第4期578-580,共3页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金重点项目(30230380);上海市科学技术委员会重大课题(04dz19504);上海市卫生局青年基金(2006Y6);复旦大学青年科学基金(08FQ37)

摘　　要：目的:探讨线粒体DNA5178A/C多态性与2型糖尿病患者糖尿病性视网膜病变(DR)的相关性。方法:将672例2型糖尿病患者分为无明显的糖尿病视网膜病变(NDR,n=518)、非增殖性糖尿病视网膜病变(NPDR,n=145)和增殖期糖尿病视网膜病变(PDR,n=9例)3组。采用聚合酶链反应-限制性片段(PCR-RFLP)技术检测线粒体DNA5178C/A多态性。评价该多态性与DR的相关性。结果:Mt5178C/A多态性在NDR、NPDR和PDR3组间基因型分布无显著性差异。结论:线粒体DNA5178A/C多态性与中国2型糖尿病患者视网膜病变无关。Objective： To observe the relationship hetween diabetic retinopathy（DR） and the mitochondrial DNA 5178 adenine/cytosine（mt5178A/C） polymorphismin in Chinese type2 diabetic patients. Methods： A case control study for a total of 672 Chinese type 2 diabetic patients which were subdivided into three groups： 518 without DR, 145 with NPDR and 9 with PDR. The mt5178A/C was genotyped by the polymerase chain reaction-restriction fragment length polymorphism method （PCRRFLP）. Results： No significant difference in genotype distribution was observed among theNDR, NPDR and PDR groups. Conclusions：The Mt5178C/A polymorphism was not associated with the DR among Chinese type2 diabetic patients.

关 键 词：2型糖尿病 糖尿病视网膜病变 线粒体DNA5178A/c 

分 类 号：R587.2[医药卫生—内分泌]