丹红注射液对体外神经元N-甲基-D-天冬氨酸损伤后的保护作用  

Protective Effects of Danhong Injection on NMDA Induced Neuronal Injury in Vitro

作　　者：吴旭青[1] 丁晶[1] 范薇[1] 

机构地区：[1]复旦大学附属中山医院神经内科,上海200032

出　　处：《中国临床医学》2009年第4期609-612,共4页Chinese Journal of Clinical Medicine

摘　　要：目的:胎鼠原代皮层神经元培养,建立N-甲基-D-天冬氨酸(NMDA)诱导的兴奋性毒性损伤模型。研究丹红注射液对于神经元NMDA损伤后的保护作用。方法:胎鼠原代神经元培养并鉴定。培养至10d时,给予不同浓度NMDA,采用MTT法检测细胞生存率并测定LDH释放率,建立NMDA损伤模型。给予两种剂量的丹红注射液干预,测定细胞生存率及LDH释放率。采用Hoechst染色及TUNEL检测小剂量丹红干预组细胞凋亡情况。结果:随着NMDA浓度的增高,神经元生存率逐步下降,LDH释放率依次增加,以NMDA300μmol·L-1组损伤最明显。丹红干预组细胞生存率提高,LDH释放率下降。小剂量丹红干预组凋亡细胞百分率减低。结论:丹红注射液可减轻NMDA诱导的体外神经元损伤,抑制神经元凋亡。Objective：To culture primary cortical neurons of embryonic mice and establish the model of N-methyl-D-aspartate （NMDA） induced neuronal injury. To study whether Danhong injection can protect neurons from excitotoxicity induced by NMDA. Methods：Primary cortical cultures were prepared from embryonic mice. Neuronal cell populations were determined. Ten days later, cultures were exposed to NMDA of various concentrations. Cell survival was evaluated by MTT assay and LDH release. The model of NMDA induced neuronal injury was established. Danhong injection of two doses was added to the culture medium with NMDA. Cell survival and LDH release were evaluated. Neuronal apoptosis of small dose Danhong intervention group was estimated by Hoechst staining and TUNEL, Results： A dose-response curve of NMDA neurotoxicity was performed and revealed the most significant reduction in MTT activity and the highest LDH release at a concentration of 300μmol/l. Danhong intervention groups had increased MTT activities and decreased LDH release. The percentage of apoptotic neurons in the small dose Danhong intervention group declined. Conclusion：Danhong injection can relieve NMDA induced neu- ronal injury in vitro and inhibit neuronal apoptosis.

关 键 词：丹红注射液 神经元 N甲基-D-天冬氨酸 凋亡 

分 类 号：R285.5[医药卫生—中药学]