梅花入骨丹多糖含量的测定  

Determination of Polysaccharide in Bauhinia Championi (Benth.) Benth.

作　　者：叶蕻芝[1,2] 杨名平[3] 林薇[1,2] 郑春松[1,4] 陈红青[3] 刘献祥[1] 

机构地区：[1]福建中医学院中西医结合研究院,福建福州350108 [2]福建省高校中西医结合基础重点实验室,福建福州350108 [3]福州大学,福建福州350108 [4]福建省中西医结合老年性疾病重点实验室,福建福州350108

出　　处：《福建中医学院学报》2009年第4期22-24,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30672701);福建省自然科学基金项目(2009J01172);福建省中西医结合老年性疾病重点实验室开放课题基金(2008J1004-27);陈可冀中西医结合发展基金(CKJ2007004;CKJ2008025;CKJ2009006)

摘　　要：目的测定梅花入骨丹多糖含量。方法采用苯酚一硫酸法。结果本法建立的标准曲线为Y=8.1249X-0.0067(r2=0.9991),检测波长为490 nm,多糖含量为2.98%,平均回收率为99.15%,RSD为1.73%(n=5)。结论此方法简便可行,重现性较好,可作为梅花入骨丹的质量评价依据,为合理开发该药提供参考。Objective To determine the polysaccharides in Bauhinia Champloni （Benth.） Benth. Methods The polysaccharides content of Bauhinia Champloni （Benth.） Benth. was determined by phenolsulfuric acid method. Results The standard curve was Y=8.1249X-0.0067 （P=0.9991）, the detection wavelength was 490 nm, the content of the polysaccharides was 2.98%, and the average recovery was 99.15% with RSD of 1.73% （n=5）. Conclusion The method is simple and reproducible and can be served as a basis for quality evaluation of Bauhinia Champloni （Benth.） Benth., and provide reference for its rational exploitation.

关 键 词：梅花入骨丹 苯酚-硫酸法 多糖 

分 类 号：R284.1[医药卫生—中药学]