虎杖愈浊汤治疗实验性大鼠慢性盆腔疼痛综合征的研究  

Study on chronic pelvic pain syndrome treated with Huzhangyuzhuo decoction

作　　者：陈国宏[1] 刘保兴[2] 李兰群[2] 王谦[3] 

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]中日友好医院中医男科,北京100029 [3]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药杂志》2009年第9期1137-1139,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药管理局专项课题项目(No.06-07LP10)

摘　　要：目的:观察虎杖愈浊汤对慢性盆腔疼痛综合征(CPPS)的治疗作用,并探讨其治疗机制。方法:将46只健康雄性Wistar大鼠随机分为分为正常组(10只)和实验组(36只),实验组采用腹腔注射百白破疫苗、多点皮内注射大鼠前列腺蛋白提纯液和福氏完全佐剂(FCA)混悬液制备CPPS大鼠模型。再将实验组大鼠随机分为模型组、中药组和西药组(每组各12只),连续给药30d后处死大鼠,取前列腺组织,观察其组织形态学改变,ELISA法检测前列腺组织匀浆IL-1β、COX-2、PGE2、β-EP的含量。结果:虎杖愈浊汤能显著减轻大鼠前列腺组织的炎症反应和组织结构的破坏程度,显著降低大鼠前列腺组织中IL-1β、COX-2、PGE2的含量,升高β-EP的含量。结论:虎杖愈浊汤对CPPS的治疗作用可能为虎杖愈浊汤能够调节前列腺组织细胞因子含量,减轻前列腺组织炎症反应和组织结构的破坏,从而对前列腺组织起到明显保护作用。Objective：To observe the therapeutic effect and mechanism of Huzhangyuzhuo decoction on treating chronic pelvic pain syndrome （CPPS）. Methods： 46 male Wistar rats were randomly divided into control group（n=10） and experiment group（n=36）. The CPPS rat model was induced by intraperitoneal injection of diphtheria-pertussis-tetanus vaccine and subcutaneous injection of purified prostate protein with FCA suspension. Rats in experiment group were subdivided into model group, Chinese medicine group and western medicine group（n=12）. After 30 days of treatment, pathology changes of prostate were observed, and levels of IL-1β, COX-2, PGE2, β-EP in prostate homogenate were measured by ELISA. Results： Huzhangyuzhuo decoction greatly reduced the inflammation of prostate and relieved the structure damage. At the same time, Huzhangyuzhuo decoction can decrease the level of IL-1β, COX-2, PGE2 and increase level of β-EP. Conclusions： Huzhangyuzhuo decoction might treat CPPS by regulating of cytokines in the prostate, depressing inflammation and relieving structure damage.

关 键 词：慢性盆腔疼痛综合征 虎杖愈浊汤 大鼠 

分 类 号：R285.5[医药卫生—中药学]