野菊花对压力负荷性大鼠左室心肌及神经内分泌因子的影响  

Influnce of wild chrysanthemum on some neuroendocrine factors in ventricular remodeling induced by abdominal aortic banding in rats

作　　者：吴琦[1] 陈长勋[1] 顾伟梁[1] 高建平[1] 

机构地区：[1]上海中医药大学药理教研室,上海201203

出　　处：《中华中医药杂志》2009年第9期1140-1143,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30572379);上海市教委课题(No.08CZ09)

摘　　要：目的:探讨野菊花对压力负荷性大鼠心室重构及神经内分泌因子的影响。方法:腹主动脉不完全结扎法(AAB)制作大鼠心肌肥厚、心室重构模型。35d药物干预后,测量大鼠收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP);称重法测定心脏指数(HW/BW、LVW/BW);放免法测定心肌血管紧张素Ⅱ(AngⅡ),血清醛固酮(ALD)、肿瘤坏死因子α(TNF-α)的水平;心肌样本碱水法测定羟脯氨酸(Hyp)的含量。结果:模型组SBP、DBP、MAP、HW/BW、LVW/BW、AngⅡ、ALD、TNF-α及Hyp含量升高(P<0.05)。野菊花改善心肌肥厚指数,降低AngⅡ、ALD、TNF-α及Hyp含量作用显著(P<0.05)。结论:野菊花通过抑制压力负荷性心室重构大鼠RAAS和交感神经系统活性,减少神经内分泌因子AngⅡ、TNF-α、ALD生成而抑制心肌肥大、心室重构,这可能是野菊花直接保护心肌,抑制心肌细胞纤维化的作用机制之一。Objective： To approach the influnce of wild chrysanthemum on some neuroendoerine factors in ventricular remodeling induced by abdominal aortic banding（AAB）in rats. Methods： Myocardial hypertrophy, ventricular remodeling model was induced by abdominal aortic banding（AAB）in rats. After 35 days＇ treatment, the systolic blood pressure（SBP）, diastolic blood pressure（DBP） and mean arterial pressure（MAP） were measured. Then the ratios of LVW/BW （left ventricle weight/ body weight）, HW/BW （heart weight/body weight）were Calculated. The Angiotensin Ⅱ（Ang Ⅱ） content in heart tissue and the Aldosterone（ALD）, Tumor Necrosis Factor （TNF- α ）concentration of blood plasma were determined by radioimmunoassay. Results： The experimental data demonstrated that wild chrysanthemum could decrease SBP, DBP, MAP and the cardiac indexes of LVW/BW and HW/BW, significantly reduce the content of Ang Ⅱ, ALD and TNF- α, decrease the Hydroxyproline content （P〈0.05）. Conclusion： Wild chrysanthemum can significantly attenuate the experimental ventricular remodeling, the mechanism may be related with its ability to inhibit the activation of rennin-angiontensin-aldosterone system（RAAS）and sympathetic nervous system（SNS）, decrease the content of neuroendocrine factors（Ang Ⅱ,ALD, TNF- α ）.

关 键 词：野菊花 心室重构 肾素-血管紧张素-醛固酮系统 羟脯氨酸 

分 类 号：R285.5[医药卫生—中药学]