对羟基苯基丙酮酸双氧化酶作用靶标的研究进展  

Progress in research of the target of p-hydroxyphenylpyruvate dioxygenase

作　　者：贺军波[1] 贺红武[1] 

机构地区：[1]华中师范大学有机合成研究所教育部农药与化学生物学重点实验室,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2009年第1期70-75,共6页Journal of Central China Normal University：Natural Sciences

基　　金：国家重点基础研究发展规划项目(2003CB114400);国家自然科学基金项目(20372023;20772042)

摘　　要：对除草剂靶标对羟基苯基丙酮酸双氧化酶(HPPD)的研究进展情况作了简要的概述,总结了植物体内HPPD的作用机制和HPPD抑制剂的作用机制,重点以NTBC为例综述了NTBC与对羟基苯基丙酮酸双氧化酶的作用研究来阐明抑制剂对HPPD酶的抑制机理.研究表明抑制剂NTBC与靶标HPPD的强亲和力主要通过两个氧原子与Fe″的二齿配位和蛋白质口袋中芳环间的π-堆积力作用,其中芳环间的π-堆积力起主要作用.The progress of research on the herbicide target of p-hydroxyphenylpyruvate dioxygenase was reviewed, and summarized the action mechanism of plant HPPD and action mechanism of HPPD inhibitor, mainly summarized the interaction of NTBC to HPPD to illustrate the mechanism of inhibitor to HPPD with NTBC as a main example. The researches indentified two binding interaction of NTBC to HPPD that might relate to its high affinity： bidentate coordination to Fe^Ⅱ and π-stacking between the aromatic ring of NTBC and two phenylalanine residues in the protein pocket, the π-stacking interactions in the protein pocket appeared to be responsible for the high affinity.

关 键 词：对羟基苯基丙酮酸双氧化酶 NTBC 作用靶标 作用机制 

分 类 号：Q554[生物学—生物化学]