腺病毒介导Canstatin基因治疗卵巢癌的实验研究  

Anti-tumor effect of canstatin by inhibition of angiogenesis in ovarian cancer in mice

作　　者：朱宝菊[1,2] 陈琛[2] 马军[2] 于泳[2] 李印[3] 

机构地区：[1]郑州大学第一附属医院,郑州450052 [2]郑州大学第二附属医院,郑州450014 [3]河南省肿瘤医院胸外科,郑州450008

出　　处：《华中师范大学学报（自然科学版）》2009年第1期117-120,共4页Journal of Central China Normal University：Natural Sciences

基　　金：河南省卫生厅科技攻关项目(200703064);河南省科技厅重点攻关项目(豫财社[2005]158号)

摘　　要：应用分子克隆技术,构建重组腺病毒质粒Adtrack-canstatin(Ad-can),并建立人卵巢上皮性癌移植瘤动物模型.动物模型建立后,将荷瘤裸鼠随机分为3组,Ad-Can、Ad-GFP和PBS对照组,每组6只.治疗采用肿瘤内局部多点注射4.0×1010PFU/ml病毒上清液或PBS液0.1 ml,共2次,注射间隔72小时.8天后肿瘤体积出现明显差异,Ad-can组明显小于对照组,P<0.01,而Ad-GFP和PBS对照组间无明显差异,P>0.05.另外,Ad-can组CD105明显减少,微血管密度降低,P<0.05.结果提示:Canstatin基因能明显抑制人卵巢上皮性癌的生长,其机制可能是通过抑制肿瘤新生血管形成起作用的.The recombinant adenovirus plasmid Adtrack-canstatin（Ad-can） was constructed by means of molecular cloning technique, and the model of ovarian cancer xenografted tumor in nude mouse were established and devided into 3 groups randomly （Ad- can, AdGFP and PBS）,6 for each group. Viral supernatant fluid or PBS were injected into tumors, 4× 1010 pfu/0, lml per injection, for a total of two injections separated by a 72-hour interval. In vivo, the tumor volume of the Ad-Can group was inhibited significantly compared with control groups from the 8th day（P〈0.01）. However, there are no obviously difference between Ad-GFP group and PBS group （P〉0.05）. In another, the CD105 expression and microvessel density（MVD） in Ad-can group was lower than that in control group （P〈0.05）. The recombinant adenovirus plasmid Ad-Can retards the growth of ovarian cancer xenografted tumors in nude mouse effectively through inhibiting the angiogenesis.

关 键 词：CANSTATIN 腺病毒 基因治疗 卵巢肿瘤 CD105 

分 类 号：Q78[生物学—分子生物学]