荧光扫描法测定复方氨酚烷胺片中胆酸含量  

Content Determination of Cholic Acid in Compound Paracetamol and Amantadine Hydrochloride Tablet by Fluorescence TLCS

作　　者：李向军 韩桂茹[2] 吴广 王玉峰 叶晓红 

机构地区：[1]河北以岭医药研究院,河北石家庄050031 [2]河北省药品检验所,河北石家庄050011 [3]药都制药集团股份有限公司,河北保定071200

出　　处：《中国药业》2009年第17期17-18,共2页China Pharmaceuticals

摘　　要：目的建立测定复方氨酚烷胺片中胆酸含量的荧光扫描法。方法用10%的硫酸乙醇溶液将胆酸增荧光后确立其荧光扫描法,并对复方氨酚烷胺片中的胆酸进行含量测定。结果增荧光后,胆酸的最佳激发波长为385nm;胆酸的点样量在0.03～0.15μg范围内与荧光强度积分值呈良好的线性关系,平均加样回收率为98.60%,RSD为1.46%(n=9)。结论所用方法简便、灵敏、重现性好、精密度高,可用于复方氨酚烷胺片的质量控制,并为其他含胆酸制剂的含量测定提供了新方法。Objective To establish a method of fluorescence TLCS for determining cholic acid in Compound Paracetamol and Amantadine Hydrochloride Tablet. Methods After the fluorescence was strengthened by alcohol solution containing 10% sulfuric acid, the method of fluorescence TLCS of cholic acid was established firstly. Then the content of cholic acid in Compound Paracetamol and Amantadine Hydrochloride Tablet was determined. Results After the fluorescence was strengthened, the best excited wavelength of cholic acid was 385 nm. The good linear correlation existed in the range of 0.03-0. 15 μg of the cholic acid applied. The average recovery rate of cholic acid （n = 9） was 98.60%, RSD was 1.46%. Conclusion The method is simple, sensitive with good repeatability and high precision. It can be used for the quality control of Compound Paracetamol and Amantadine Hydrochloride Tablet and provides a new method to determine the content of cholic acid in other preparations.

关 键 词：荧光扫描法 复方氨酚烷胺片 胆酸 最佳激发波长 

分 类 号：R927.2[医药卫生—药学] R971.1