罗格列酮钠对葡萄糖胺诱导的HIT-T15细胞超微结构损伤的保护作用  

Protection of Rosiglitazone Sodium Against HIT-T15 Cell Damage Induced by Glucosamine

作　　者：张新霞[1,2] 童南伟[1] 黎瑶[1,3] 

机构地区：[1]四川大学华西医院,四川成都610041 [2]成都中医药大学西内教研室,四川成都610075 [3]成都医学院附属医院,四川成都610036

出　　处：《亚太传统医药》2009年第8期19-23,共5页Asia-Pacific Traditional Medicine

摘　　要：目的:观察罗格列酮钠(RSGN)对葡萄糖胺(GLcN)诱导的HIT-T15细胞超微结构损伤及其保护作用。方法:以叙利亚仓鼠胰岛β细胞株HIT-T15细胞作为研究对象,实验分组:正常对照组;GLcN+高浓度葡萄糖培养组;GLcN+高浓度葡萄糖+RSGN10-6M培养组;GLcN+高浓度葡萄糖+RSGN10-8M培养组。各组培养48h,透射电镜观察HIT-T15细胞线粒体超微结构。结果:透射电镜观察HIT-T15细胞,GLcN组可见凋亡细胞及较多的坏死细胞,多数线粒体模糊不清,个别线粒体有肿胀,核周隙变宽内质网空泡变性的病理变化;RSGN10-8M组的HIT-T15细胞超微结构病变明显减轻,与RSGN10-8M组比较,RSGN10-6M组病变无明显改变。结论:GLcN可使胰岛β细胞株HIT-T15细胞超微结构损伤,RSGN可部分地阻止这种损伤的形态改变,且和其剂量有一定关系。Objective： The present study was to study the protective effect of rosiglitazone sodium （RSGN） on pathologic changes of HIT-T15 cells uhrastructure induced by glucosamine. Methods： HIT-T15 cells were used and their uhrastructure was studied with transmission electron microscope. The cells were divided into four groups： （1） control, （2）SmMGLeN＋llmMglucose, （3） 8mMGLcN ＋ 11 mMglucose＋ RSGN10 6 M and （4） 8mMGLcN ＋ 11mMglucose ＋ RSGN10.8 M. Result： Transmission electro-tele- scope showed apoptosis, necrosis and endoplasmic reticulums vaculation in HIT-T15 ceils treated in GLcN, with a characteriza- tions of illegible and swelling mitochondrion, wide intermittent space of nuclear, and degenerated endoplasmic reticulum. The HIT-T15 cells treated with RSGN10-SM were greatly improved in the pathological alterations of uhrastructure. Conclusion： Our findings indicated that HIT-T15 cells were damaged by GLcN, while RSGN protected the cellular structures against the damage in a concentration-dependent manner.

关 键 词：葡萄糖胺 罗格列酮钠 细胞线粒体 超微结构 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]