肉苁蓉中异麦角甾苷的制备及含量测定  

Preparation and Quantitative Analysis of Acteoside Isome Standard Sample in Cistanche

作　　者：杨杰[1] 白雪[1] 肖海涛[1] 张一[1] 刘昌福[1] 吴文利[1] 郝小燕[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004

出　　处：《亚太传统医药》2009年第8期54-56,共3页Asia-Pacific Traditional Medicine

基　　金：贵州省自然科学技术基金项目(黔科合J字[2008]2068号)

摘　　要：目的：研究中药肉苁蓉中异麦角甾苷标准品的制备及含量测定方法。方法：采用微晶纤维素色谱与半制备高效液相色谱组合分离技术，分离纯化异麦角甾苷，采用薄层色谱法和高效液相色谱面积归一化法检测纯度，波谱法鉴定其结构，反相高效液相色谱法测定其含量。结果：制备的化合物鉴定为异麦角甾苷，纯度为98．6％；含量测定在0．21841．308 μg，呈良好线性关系，r=0．9995，平均回收率为102．8％，RSD为2．60％，n=6。结论：该制备方法效率高，所得化合物纯度高，可用于大量制备；含量测肉苁蓉，定方法准确，简便，可靠，重复性好。Objective： To study a preparation and determination method of isoacteoside in Herba Cistanches. Methods： The isoac- teoside was separated and purified by semi-preparative high performance liquid chromatograph and microcrystalline cellulosese chromatography. Its purity was determined by TLC and HPLC analysis and normalization of peak areas. The structure of isoacte- oside was identified by spectrum, and the quantification was determined by RP-HPLC. Results： The compound was identified as isoacteoside. The purity of the isoacteoside was 98.60%. There was a good linearity between 0. 218 μg-1. 308μg （r = 0. 9995）. The average recovery was 102.8%（RSD = 2. 60%, n = 6）. Conclusion： This preparation method is quick and convenient for pro- ducing the pure compounds from herbs with large capacity quantitative. The method is accurate and simple with good reproduc- ibility, and it can control the quality of this herb and preparation effectively.

关 键 词：肉苁蓉 异麦角甾苷 半制备高效液相色谱法 反相高效液相色谱法 含量测定 

分 类 号：R284[医药卫生—中药学]