AcSDKP对正常人真皮成纤维细胞表达TGF-β1的影响  

Effects of AcSDKP on TGF-β1 Expression in Human Dermal Fibroblasts

作　　者：邓智明[1] 赵喜彬[1] 郑江红[1] 邓辰亮[1] 丁志[1] 茅广宇[1] 王惠东[1] 杨松林[1] 

机构地区：[1]上海交通大学医学院附属第六人民医院整形外科,200233

出　　处：《组织工程与重建外科杂志》2009年第4期195-198,共4页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金(30571929);上海市卫生局资助课题(2006055)

摘　　要：目的探讨N-乙酰基-丝氨酰-天冬氨酰-赖氨酰-脯氨酸(AcSDKP)对真皮成纤维细胞表达TGF-β1的影响。方法对人皮肤成纤维细胞原代培养及鉴定后,给予不同浓度AcSDKP(10-7mol/L、10-8mol/L、10-9mol/L、10-10mol/L、10-11mol/L)干预,设立空白对照组。WST-8法检测人皮肤成纤维细胞增殖;RT-PCR技术检测人皮肤成纤维细胞TGF-β1mRNA表达;酶联免疫法检测培养细胞上清液TGF-β1含量。结果与空白对照组相比,各浓度组对成纤维细胞的增殖都有抑制作用,其中10-10mol/LAcSDKP抑制成纤维细胞增殖作用最强;10-7mol/L、10-8mol/L、10-9mol/L、10-10mol/LAcSDKP对TGF-β1mRNA表达有显著的抑制作用,且各浓度组间的抑制作用无差异;10-8mol/L、10-9mol/L、10-10mol/LAcSDKP可使培养上清液中的TGF-β1显著减少,各浓度组间差异无显著性。结论AcSDKP能够抑制人皮肤成纤维细胞增殖及TGF-β1的生成,有望成为病理性瘢痕防治的新方法。Objective To study the effects of N-acetyl-seryl-aspartyl-lysyl-praline （AcSDKP） on TGF-β1 synthesis in human dermal fibroblasts. Methods Human dermal fibroblast after Primary cultures and identification were subjected to AcSDKP （10^-7 mol/L, 10^-8 mol/L, 10-9 mol/L, 10^-10 mol/L, 10^-11 mol/L, respectively） treatment. The control group was not seeded AcSDKP. The proliferation of human dermal fibroblasts were detected by WST-8. The expression of TGF-β1 mRNA were analyzed with RT-PCR. TGF-β1 in the supernatant were determined by enzyme-linked immunosorbent assay. Results Compared with control group, the proliferation of human dermal fibroblasts were inhibited by all AcSDKP groups, and 10^-10 mol/L AcSDKP had the most effects; 10^-7 mol/L, 10^-8 mol/L, 10^-9 mol/L, 10^-10 mol/L AcSDKP significantly inhibited the expression of TGF-β1 mRNA, and the different groups had no different effects; Expression of TGF-β1 in the supernatant were significantly inhibited by 10^-8 mol/L, 10-9 mol/L, 10^-10 mol/L AcSDKP. Conclusion The proliferation and expression of TGF-β1 in human dermal fibroblasts were significantly inhibited by AcSDKP. AcSDKP has the best preventive effect on the pathological scar.

关 键 词：N-乙酰基-丝氨酰-天冬氨酰-赖氨酰-脯氨酸 真皮成纤维细胞 转化生长因子-Β1 表达 

分 类 号：R619.6[医药卫生—外科学]