EMS诱发大豆“南农94-16”突变体库的扩建及部分突变体的SSR分析  

Construction of Mutant Pools for Soybean "Nannong 94-16" Induced by EMS and Analysis of SSR Marker on Several Mutants

作　　者：陈远东[1] 喻德跃[1] 

机构地区：[1]南京农业大学大豆研究所国家大豆改良中心作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《大豆科学》2009年第4期574-577,582,共5页Soybean Science

基　　金：国家重点基础研究发展计划(973计划)资助项目(2004CB117206);国家高技术研究发展计划(863计划)资助项目(2006AA10Z1C1)

摘　　要：甲基磺酸乙酯(EMS)是一种稳定、高效的诱发点突变的诱变剂。采用EMS处理大豆"南农94-16"的种子,在初步构建大豆突变体库的基础上进一步扩建了大豆突变体库,获得95份茎、叶、花、种子等性状变异的突变材料。采用200对SSR标记分别对其中高蛋白、叶黄化等9个不同性状的突变体进行了鉴定。结果表明:与对照相比突变体发生了多位点的变异。构建的突变体库不但可以作为新的种质资源,而且可促进大豆功能基因组研究的开展。EMS is a stable and effective mutagen. In this study, soybean mutant pools were constructed by treating the seeds of soybean＂ Nannong 94-16＂ with EMS. These pools include 95 mutants of stem, leaf, cotyledon,flower and seed. In addition,200 SSR primer pairs were used to characterize several mutants such as high-protein content mutant,revealing polymorphism between mutants and base materials. These mutant pools created for＂ Nannong 94-16＂ will be valuable for the research in breeding and functional genomics soybean.

关 键 词：EMS 大豆 突变体 SSR 

分 类 号：S565.1[农业科学—作物学]