不同形态氮对大豆根瘤生长及固氮的影响  

Effect of Different Forms Nitrogen on Nodule Growth and Nitrogen Fixation in Soybean(Glycine max L.)

作　　者：严君[1,2] 韩晓增[1,2] 王守宇[1] 王树起[1] 李晓慧[1] 

机构地区：[1]中国科学院东北地理与农业生态研究所,黑龙江哈尔滨150081 [2]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2009年第4期674-677,共4页Soybean Science

基　　金：国家科技支撑计划资助项目(2006BAD21B01;2006BAD05B05);黑龙江省科技攻关资助项目(GB06B101-1-4)

摘　　要：采用框栽的培养方法,通过施用不同形态氮肥,探讨不同形态氮对大豆根瘤生长、含氮量、氮积累量以及固氮酶活性的影响。结果表明:不同形态的氮源,对根瘤干重、数量有促进作用,同时增加了根瘤中的含氮量和氮积累量。根瘤干重及数量的变化均为铵态氮(N2)>蛋白氮(N4)>硝态氮(N1)>氨基酸态氮(N3)>酰胺态氮(N5)>生物固氮(N0),其中N0处理的根瘤干重、数量最少,而N2处理的根瘤干重和数量最多。根瘤含氮量的变化为N1>N4>N5>N2>N3>N0,其中N1处理下的根瘤中的根瘤含氮量最高,N1、N4和N5各处理与N0处理间差异显著(P<5%)。而氮积累的变化为N2>N4>N1>N5>N3>N0,各处理与N0处理相比,差异极显著(P<1%),与含氮量的变化趋势略有不同。添加不同形态的氮源对根瘤固氮酶活性有抑制作用,其变化趋势为N0>N5>N3>N2>N4>N1,N0处理的固氮酶活性最高。Nitrogen plays an important role in nitrogen fixation of soybean. In this paper, a pot culture experiment was carded out to study the effects of different forms nitrogen on the variation of dryweight （DW）, number, nitrogen content, nitrogen accumulation and nitrogenase activity in nodule of soybean （ Glycine max L. ） at flowering stage. The results showed that dif- ferent forms nitrogen promoted nodule formation and growth, which embodied in dry weight and number significantly increased compared with biological nitrogen fixation. The order of the nodule DW and nodule number were NO4 -N（N2） 〉 Protein-N （ N4 ） 〉 NOr -N （ N1 ） 〉 Amino Acid-N （ N3 ） 〉 Amide-N （ N5 ） 〉 Biological Nitrogen Fixation （ N0）, the least nodule DW and number appeared in NO treatment,while the most occurred in N2 treatment. Meanwhile different forms nitrogen increased N content and N accumulation in nodule. The trend of the nitrogen content in nodule was as follows ： N1 〉 N4 〉 N5 〉 N2 〉 N3 〉 NO, and nitrogen content was the highest in the N1 treatment, moreover, the difference among NO, NI, N4,3/5 was significan at 5% level. However,the order of the N accumulation was N2 〉 N4 〉 N1 〉 N5 〉 N3 〉 NO. The different forms nitrogen remarkably affected nitrogenase activity, the trend was NO 〉 N5 〉 N3 〉 N2 〉 N4 〉 N1, and the nitrogenase activity was the highest in NO treatment. Therefore, different forms nitrogen had different effects on nitrogen fixation of soybean.

关 键 词：根瘤 固氮酶活性 不同形态氮 大豆 

分 类 号：S565.1[农业科学—作物学]