基于分子标记的油松种群遗传保护分析  

Genetic Conservation of Pinus tabulaeformis Population Implicated by Molecular Markers

作　　者：李毳[1] 柴宝峰[2] 

机构地区：[1]山西财经大学环境经济学院,太原030006 [2]山西大学生物技术研究所,太原030006

出　　处：《应用与环境生物学报》2009年第4期459-463,共5页Chinese Journal of Applied and Environmental Biology

基　　金：山西省回国留学人员科研基金(No.200846);国家自然科学基金(No.30770294)资助~~

摘　　要：利用分子标记技术分析了山西5个天然油松种群140个个体的遗传特征,比较了不同种群的多态位点显性频率、遗传多样性指数、基因流和分化系数.筛选的5个ISSR引物和5个RAPD引物共扩增到位点68个。Pinus tabulaeformis widely distributes in China from northeast to southwest, and plays an important rote in natural forests and artificial plantations. Its genetic characteristics of DNA in 140 samples from 5 populations in Shanxi were investigated by using molecular technique. A total of 68 reproducible bands were identified using 5 RAPD primers and 5 ISSR primers, and 50 bands of them were polymorphicl loci. The statistic analysis of the genetic parameters indicated that gene frequency, genetic diversity index and the relative magnitude of differentiation among populations were 0.000 0-1.000 0, 0.020 0-0.500 0 and 0.003 7-0.615 6, respectively, suggesting that the genetic diversity ofP. tabulaeformis populations in Shanxi mainly lies on different allelic frequencies and on some high level genetic differentiating locus. Based on the characteristics of P. tabulaeformis populations in Shanxi, it is suggested that the genetic resource of pine can only be conserved well by enhancing the protecting ofP. tabulaeformis habitats. Fig 2, Tab 4, Ref 16

关 键 词：油松 分子标记 遗传多样性 种群结构 遗传保护 

分 类 号：S791.254.01[农业科学—林木遗传育种]