芳香化合物羟基化酶研究进展  

Advances in Hydroxylation of Aromatic Compounds with Oxygenase

作　　者：张强[1] 曲媛媛[1] 周集体[1] 苟敏[1] 皮文清[1] 

机构地区：[1]大连理工大学环境与生命学院工业生态与环境工程教育部重点实验室,大连116024

出　　处：《应用与环境生物学报》2009年第4期540-545,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.50608011)资助~~

摘　　要：芳香族化合物的选择性羟基化是合成化学中最具挑战性的化学反应之一,特别是由于近年来羟基芳香族化合物在医学上作为前驱体使用,使其越来越受到重视.通过单一酶或者整个微生物细胞进行的生物催化氧传递,是完成芳香化合物选择性羟基化反应的一种有效方式,不仅在受污染环境的生物修复中作用重大,而且还可以替代并拓宽传统的化工合成工艺,广泛用于化工中间体的生产,这使得芳香化合物羟化酶成为重要的工业用酶之一.本文综述了芳香化合物羟基化酶的种类、作用机理及工业应用等方面的最新研究进展.Aromatic oxygenases are able to catalyze the selective hydroxylation of aromatic compounds, and the hydroxylation products could be used as the major chemical intermediates. Therefore, these oxygenases are becoming the main objects in the fields of biotechnology and chemistry. Selective hydroxylation of aromatic compounds is among the most challenging chemical reactions in synthetic chemistry and has gained steadily increasing attention during recent years, particularly because of the use of hydroxylated aromatics as precursors for pharmaceuticals. Biocatalytic oxygen transfer by isolated enzymes or whole microbial cells is an elegant and efficient way to achieve selective hydroxylation. It plays an important role not only in the bioremediation of the polluted environment, but also in chemical synthesis. Those enzymes are one of the important industrial enzymes, and they can be applied in the production of oxidative materials directly or indirectly and can overcome many problems of chemical synthesis. The recent advances in the studies on the aromatic hydroxylation oxygenases, and their mechanism and application in industry are summarized in this paper. Fig 5, Tab 1, Ref42

关 键 词：加氧酶 过氧化物酶 P450 非血红素铁加氧酶 羟基化 

分 类 号：Q55[生物学—生物化学]