生脉注射液对百草枯中毒大鼠肺组织核因子-кB以及诱导型一氧化氮合酶表达的影响  

Effect of Pulse-activating Injection on the Expression of Nuclear Factor-kappa B and iNOS in Lung Tissue of Acute Paraquat Poisoned Rats

作　　者：刘会芳[1] 赵燕燕[1] 魏芳[1] 

机构地区：[1]河北大学医学部卫生职业技术学院,河北保定071000

出　　处：《时珍国医国药》2009年第8期1930-1932,共3页Lishizhen Medicine and Materia Medica Research

基　　金：河北省中医药管理局科研基金资助项目(No.2008070;2008072);河北省卫生厅科研基金资助项目(No.08370);河北省科技攻关项目(No.05276101D-88;42761220);河北大学医学部科学研究基金资助项目(No.2007Q06)

摘　　要：目的观察百草枯(PQ)急性中毒大鼠所致肺损伤(ALI)时核因子-кB和诱导型一氧化氮合酶(iNOS)的变化,探讨生脉对急性百草枯中毒所致肺损伤的保护作用。方法将50只SD大鼠随机分成5组,空白组、阴性对照组、阳性对照组、生脉低剂量组和生脉高剂量组。观察大体标本组织病理,同时测定肺组织核因子-кB和iNOS活性。结果与阴性对照组相比,生脉低剂量组肺组织病理显示肺淤血、肺水肿明显减轻;核因子-кB和iNOS也降低(P<0.05或P<0.01)。结论核因子-кB及iNOS在百草枯所致大鼠肺损伤中起重要作用,生脉注射液能降低核因子-кB及iNOS水平,减轻百草枯中毒大鼠肺组织损伤。Objective To invesigate the protective effect of pulse - activating injection against rat acute lung injury. Methods 50 Spragne- Dawley （SD） rats were randomly divided into five experimental groups： blank group, negative control group, positive control group, Pulseactivating injection low dose group（LDG）, Pulse -activating injection high dose group（HDG）. Macroscopic and histopathological examinations were performed, and the activities of lung tissue homogenate NK - κB and iNOS were measured. Results Compared with negative control group, and biological indexes of lung injury and the contents of NK - κB and iNOS were significantly decreased in pulse - activating injection groups （ P 〈 0.05, P 〈 0.01 ）. Conclusion NK - κB and iNOS play important roles in lung injury of poisoned rats. Pulse - activating injection can ameliorate the lung injury and decrease the levels of NK - κB and iNOS.

关 键 词：生脉注射液 百草枯 肺损伤 核因子-κB 一氧化氮 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]