猪SP-A基因荧光定量PCR检测方法的建立  

Development of FQ-PCR Assay for Detecting Porcine SP-A Gene

作　　者：乔莉娟[1,2] 王立贤[1] 颜华[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]邯郸学院生物科学系,河北邯郸056005

出　　处：《动物医学进展》2009年第8期24-29,共6页Progress In Veterinary Medicine

基　　金：国家"十一五"科技支撑计划项目(2006BAD01A08);国家高技术研究发展计划项目(2006AA10Z199)

摘　　要：SP-A(surfactant protein-A)mRNA的表达对肺的发育、成熟均起关键作用,并且与肺部疾病的发生相关。为了建立荧光定量PCR技术检测猪SP-A表达量的方法,根据猪SP-A基因的mRNA序列(EU622632)设计引物,从纯化模板、PCR程序设计等方面进行了优化,建立了基于SYBR Green I染料技术的Power SYBR(Green荧光定量PCR方法。结果表明,所建立的Power SYBR(Green荧光定量PCR方法检测猪SP-A基因具有特异性好、简便、可靠等特点,为猪SP-A基因定量分析奠定了基础。The expression of surfactant protein A mRNA is key to development and maturity of lungs, and is related to nosogenesis of pulmonary disease. The aim of this study was to establish a FQ-PCR method for porcine SP-A expression. According to the mRNA sequence of SP-A （EU622632）, primers of porcine SP-A gene were designed and a Power SYBR Green FQ-PCR method based on SYBR Green I dye was de- veloped. The results showed that the Power SYBR Green FQ-PCR method established in the study had advantages of excellent specificity, convenience, reliability, and so on. The results of this study provided useful methodological basis for quantitative analysis of porcine SP-A gene.

关 键 词：猪 SP—A基因 荧光定量PCR 

分 类 号：Q78[生物学—分子生物学] S858.28[农业科学—临床兽医学]