杂种落叶松未成熟胚的体细胞胚发生和植株再生  

Somatic Embryogenesis and Plantlet Regeneration from Immature Zygotic Embryos of Hybrid Larch

作　　者：王伟达[1] 李成浩[1] 杨静莉[1] 张含国[1] 张淑玲 

机构地区：[1]东北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150040 [2]抚顺矿业集团有限责任公司林业处,抚顺113008

出　　处：《林业科学》2009年第8期34-38,183,共6页Scientia Silvae Sinicae

基　　金：黑龙江省科技攻关重点项目(GB06B303-6);国家自然科学基金项目(30671701)

摘　　要：以未成熟合子胚为外植体,建立杂种落叶松胚性愈伤组织诱导和植株再生体系。从日本落叶松5×长白落叶松77-3和日本落叶松5×兴安落叶松9两个家系诱导出胚性愈伤组织,授粉后65天左右采集的合子胚在添加0.5mg·L-12,4-D+0.5mg·L-16-BA+0.5mg·L-1KT的S培养基上胚性愈伤组织诱导率最高,为10%。从胚性愈伤组织中筛选出3个具有稳定分化能力的胚性细胞系,其中授粉后65天采集的日本落叶松5×长白落叶松77-3合子胚诱导的胚性细胞系的每克胚性愈伤组织形成的体胚数、体胚萌发率、植株再生率最高,分别为18.1个,77.0%,28.1%。再生植株的移栽成活率为41.8%。This paper reported that an in vitro embryogenic callus induction and plantlet regeneration system from immature zygotic embryos of hybrid larch was established. Embryogenie calli were induced from zygotic embryos of Larix kaempferi 5×L. olgensis 77-3 and Larix kaempferi 5×L. gmelinii 9. The embryogenic callus induction rate was the highest when zygotic embryos were collected 65-days after pollination and cultured on S medium supplemented with 0.5mg·L^-1 2,4-D ＋ 0.5 mg·L^-1 6-BA ＋ 0.5 mg·L^-1 KT, with 10% embryogenic callus induction rate. Three embryogenic cell lines were found to have stable regenerating ability. The cell lines from zygotic embryos of L. kaempferi 5×L. olgensis 77-3 collected 65-days after pollination were the best, with 18.1,77.0%, and 28.1% of number of somatic embryo per gram embryogenie callus, germination rate and plant regeneration rate, respectively. Up to 41.8% of the transplanted plantlets were successfully survived in soil.

关 键 词：杂种落叶松 胚性愈伤组织 合子胚 组织培养 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]