枣高密度遗传图谱的构建与树干直径的QTL分析  

Construction of a Dense Genetic Linkage Map and QTL Analysis of Trunk Diameter in Chinese Jujube

作　　者：齐靖[1] 董祯[2] 毛永民[1] 申连英[1] 张玉星[1] 刘杰[1] 王晓玲[1] 

机构地区：[1]河北农业大学园艺学院,保定071001 [2]河北女子职业技术学院,石家庄050091

出　　处：《林业科学》2009年第8期44-49,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金资助项目(30370994);"十一五"国家科技支撑计划资助项目(2006BAD01A1701);河北省自然科学基金资助项目(C2007000521)

摘　　要：以冬枣×临猗梨枣杂交F1代的150株个体为作图群体,利用AFLP标记和RAPD标记,对已有的枣遗传连锁图谱进行加密,构建1张包含388个AFLP标记和35个RAPD标记、由15个连锁群组成的高密度枣遗传连锁图谱,覆盖基因组总长度1309.4cM,标记间平均距离3.1cM。与原图谱相比,总图距增加72cM,标记间平均距离缩短0.8cM,大于10cM的标记间隔减少7个,图谱饱和度显著提高。在新构建图谱的基础上,利用JionmapQTL4.0软件,首次对控制枣树干直径性状的QTL进行分析。共检测到控制枣树干直径的QTL6个,分布在5个连锁群上,分别可解释38.1%,10.0%,14.4%,10.1%,13.4%和31.0%的表型变异值。In this study,AFLP and RAPD markers were used to increase density of the genetic linkage map of Chinese jujube based on a Ft population of 150 individuals derived from the cross between Dongzao （ Zizyphus jujuba ‘Dongzao＇） and Linyilizao （ Zizyphus jujuba ‘Linyilizao＇）. As a result, a dense genetic linkage map of Chinese jujube was constructed, and it consisted of 15 linkage groups and covered 1309.4 cM,iucluding 388 AFLP markers and 35 RAPD markers. The average interval distance was 3.1cM. Compared with the previous linkage map, the total length of map increased by 72 cM, the average interval distance reduced by 0.8 cM, and the number of intervals with the distance 〉 10 cM between adjacent markers declined 33 %. Based on the newly constructed genetic linkage map, the QTLs for trunk diameter in jujube was also analyzed in this study, and a total of 6 QTLs were identified, and they could explain 38.1%, 10.0%, 14.4%, 10.1%, 13.4% and 31.0% phenotypic variation respectively.

关 键 词：枣 AFLP标记 RAPD标记 遗传图谱 树干直径 QTL分析 

分 类 号：S718.46[农业科学—林学] Q943.2[生物学—植物学]