槭属2品种叶变色期花青苷含量与相关酶活性的变化  

Anthocyanin Content and the Relevant Enzymes Activities during Leaf Color Changing of Two Acer Species

作　　者：冯立娟[1] 苑兆和[1] 尹燕雷[1] 招雪晴[1] 许鑫科[2] 徐榕[2] 李自峰 

机构地区：[1]山东省果树研究所,泰安271000 [2]山东农业大学林学院,泰安271018 [3]山东林业科技培训中心,济南250013

出　　处：《林业科学》2009年第8期56-60,共5页Scientia Silvae Sinicae

基　　金：山东省农业良种产业化项目;山东省农业科学院高技术自主创新项目

摘　　要：以自由人槭‘秋天火焰’和红花槭‘白兰地’为试材,研究槭属品种变色过程中(10月12日—11月19日),叶片花青苷含量及其生物合成相关酶苯丙氨酸解氨酶(PAL)、查儿酮异构酶(CHI)、二氢黄酮醇还原酶(DFR)和类黄酮糖基转移酶(UFGT)的活性变化。结果表明:2个品种叶片中花青苷含量随叶色的变化为单峰曲线,分别在第20和25天出现峰值;PAL,DFR和UFGT酶活性与花青苷含量均成2次曲线关系,CHI酶活性与花青苷含量呈极显著的线性关系;PAL,CHI和UFGT是‘秋天火焰’花青苷合成的关键酶,CHI和UFGT是‘白兰地’花青苷合成的关键酶。Content of anthocyanin and the activities of enzymes in anthocyanin biosyntheses including plenylalanine ammonialyase （PAL）, chalcone ismoerase （CHI）, dihydroflavonol reductase （DFR） and UDP glucose-flavonoid-3-O-glycosyhranfe-rase（UFGT） were investigated in Acer×freemanii ‘Autumn Blaze＇ and Acer rubrum ‘Brandywine＇ during the leaf color-changing period. The results indicated that the changes of anthocyanin content exhibited a single peak curve in the two American maple leaves, and the peak appeared at the twentith and twenty-fifth, respectively. The activity of PAL, DFR and UFGT was quadratically correlated with the content of anthocyanin, while CHI activity was significantly and positively correlated with the content of anthocyanin. PAL, CHI and UFGT were the key enzymes in anthocyanin synthesis in ‘Autumn Blaze＇ leaves. CHI and UFGT were the key enzyme in anthocyanin synthesisin ‘＇Brandywine＇ leaves.

关 键 词：槭属 花青苷 苯丙氨酸解氨酶 查儿酮异构酶 二氢黄酮醇还原酶 类黄酮糖基转移酶 

分 类 号：S718.43[农业科学—林学]