翠菊‘花束绯红’下胚轴体胚发生及植株再生  

Somatic Embryogenesis and Plantlet Regeneration of Hypocotyl Explants of Callistephus chinensis ‘Bouquet Scarlet’

作　　者：杜丽[1] 周索[1] 杨建伟[1] 喻亚丽[1] 谢艳华[1] 包满珠[2] 

机构地区：[1]南阳师范学院生命科学与技术学院,南阳473000 [2]华中农业大学园艺林学学院教育部园艺植物生物学重点实验室,武汉430070

出　　处：《林业科学》2009年第8期166-169,F0003,共5页Scientia Silvae Sinicae

基　　金：河南省教育厅科技攻关项目(0811033300);南阳师范学院高层次人才科研启动费资助项目(nynu200746)

摘　　要：翠菊（Callistephus chinensis）是当今世界主要一、二年生花卉之一、又是我国原产草花中之佼佼者。自1728年由我国传至欧洲然后遍栽世界各地后，至今风靡不衰（陈俊愉，2001）。然而，在翠菊的栽培过程中病虫害严重（穆红等，2004），不耐盐碱（刘淑贤，2002），鲜切花瓶插寿命短，This paper reported the successful embryogenic callus induction and somatic embryo plantlet regeneration from hypocotyls of CaUistephus chinensis ‘Bouquet Scarlet＇. Experimental results indicated that plant hormone types and their concentrations, carbohydrate sources and sucrose concentrations had varied effect on embryogenic callus induction from hypocotyls. The appropriate medium for embryogenic callus induction of C. chinensis‘Bouquet Scarlet＇ was MS ＋ 0.5 mg·L^-1 6-BA ＋ 0.5 mg·L^-1 IBA, and with the medium the embryogenic callus induction rate was 27.1%. Sucrose at 30 g·L^-1 was the suitable carbohydrate source and concentration. The suitable medium for planflet regeneration of C. chinensis ‘Bouquet Scarlet＇ embryogenic calli was MS ＋ 0.5 mg·L^-1 6-BA ＋ 0.5 mg·L^-1 IBA ＋ 550 mg·L^-1 proline, and with the medium the plantlet regeneration rate was 46.7 %.

关 键 词：翠菊 下胚轴 胚性愈伤组织 植株再生 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]