大肠埃希菌中质粒AmpC酶的流行分布  

Distribution of plasmid-mediated AmpC beta-lactamase in Escherichia coli

作　　者：李轶[1] 周绪华[1] 冯羡菊[2] 

机构地区：[1]河南省人民医院检验科,河南郑州450003 [2]郑州大学第一附属医院检验科,河南郑州450003

出　　处：《检验医学》2009年第8期595-597,共3页Laboratory Medicine

基　　金：河南省医学科技攻关项目(200703033)

摘　　要：目的了解河南地区大肠埃希菌中质粒AmpC酶的流行分布及其基因特征。方法对380株大肠埃希菌进行AmpC酶表型筛选,对筛选阳性株进行多重聚合酶链反应(PCR)及型特异PCR、克隆、测序;等电聚焦电泳检测细菌β-内酰胺酶等电点;接合实验验证质粒传递性;肠杆菌科基因组内重复一致序列(ERIC-2)PCR检测同源性。结果380株大肠埃希菌中2.1%(8株)的细菌产AmpC酶,经测序证实均为DHA-1型质粒AmpC酶基因,ERIC-2 PCR结果显示质粒AmpC酶主要以非克隆传播为主。结论河南地区发现DHA-1型质粒AmpC酶以非克隆传播为主。Objective To investigate the genotype and distribution of plasmid-mediated AmpC β-1actamases in Escherichia colt in Henan. Methods 380 Escherichia colt were detected by standard disc diffusion method which was used as screen test for AmpC β-lactamase. Multiplex polymerase chain reaction （PCR） , type-specific PCR, clone, sequencing, isoelectric focusing analysis, conjugation and enterobacterial repetitive intergenic consensus-2 （ERIC-2） PCR were performed on positive strains to determine the genotype, the isoelectric point, the transitivity of plasmid and the homology of AmpC β-1actamases. Results 8 of 380 Escherichia colt strains （2. 1% ） were AmpC β-1actamasespositive strains. All of them were blaDnA which were confirmed by sequencing. The results of ERIC-2 PCR analysis indicated that the prevalences of plasmid-mediated AmpC β-1actamase were not due to epidemic strains. Conclusions The clinical isolate of Escherichia colt producing DHA-1 type plasmid-mediated AmpC β-1actamase was found in Henan. The prevalence were not due to epidemic strains.

关 键 词：DHA-1 质粒 AMPC酶 大肠埃希菌 

分 类 号：R378.2[医药卫生—病原生物学]