三分群血细胞分析仪显微镜复检标准的制定及临床应用  

Criteria formulation and its clinical application of microscopic retest by three-part differential hemocytes analyzer

作　　者：申志红[1] 杨宇溪[1] 

机构地区：[1]云南省玉溪市人民医院检验科,653100

出　　处：《检验医学与临床》2009年第10期751-752,754,共3页Laboratory Medicine and Clinic

摘　　要：目的建立三分群血细胞分析仪显微镜复检的标准,保证血细胞分析检验结果的准确性。方法根据血细胞分析仪的特点制定显微镜复检的标准,通过对1029份标本的数据分析,评价标准的可靠性。结果符合血涂片复检标准的标本中,白细胞计数异常、血红蛋白及红细胞异常、平均红细胞体积及红细胞分布宽度异常、白细胞分类及直方图异常和血小板计数及直方图异常分别占96.7%、97.9%、96.6%、72.1%和66.9%。镜检结果与血细胞分析仪检测结果的一致性程度(Kappa值)分别为0.98、0.99、0.98、0.74和0.73。仪器检测的敏感度为87.1%,特异度为95.9%,准确性为93.1%。结论制定血细胞显微镜复检标准可增加血细胞检测准确性,为临床提供更有价值的检验信息。Objective To establish the criteria of microscopic retest by three-part differential hemocytes analyzer for ensuring the accuracy of hemocytes analyzing results. Methods To formulate the microscopic retest criteria based on the characteristics of hemocytes analyzer and to evaluate its feasibility of the criteria by 1029-sample data analysis. Results The samples according with blood smear retest criteria demonstrated that the abnormity in leucocytes counting,hemoglobin and erythrocytes, average erythrocyte volume and erythrocyte distribution width, leucocytes classification and its histogram, blood platelet counting and its histogram accounted for 96. 7%, 97. 70%0,96. 6 % ,72.1 % and 66.9 % respectively. The consensus degree （Kappa value） between the analyzer examination results and the microscopic examination results was 0. 99,0. 98,0. 98,0. 74 and 0. 73 respectively. The sensitivity was 87.1 % ,the specificity was 95.9 %and the accuracy was 93.1 % by analyzer examination. Conclusion To formulate the criteria of bemocytcs microscopic retest can increase the hemocytes examination accuracy and supply more valuable information for clinic.

关 键 词：血细胞 血细胞分析仪 显微镜检查 复检标准 

分 类 号：R446.11[医药卫生—诊断学]