新城疫疫苗对雏鸡支气管结合淋巴组织形态发育的影响  

THE EFFECT OF NEWCASTLE DISEASE VACCINE GIVEN BY OCULAR ROUTE TO THE BRONCHUS-ASSOCIATED LYMPHOID TISSUE (BALT) IN CHICKENS

作　　者：孙斌[1,2,3] 张维国[1,2,3] 王德利[1,2,3] 王守岩 梁洪君[1,2,3] 

机构地区：[1]黑龙江八一农垦大学动物科技学院 [2]黑龙江省齐齐哈尔市建华区兽医防疫站 [3]黑龙江省勤得利农场

出　　处：《黑龙江八一农垦大学学报》1998年第2期59-61,共3页journal of heilongjiang bayi agricultural university

摘　　要：５０只１日龄雏鸡随机分成数量相等的免疫组和非免疫组。免疫组雏鸡于１日龄用鸡新城疫Ⅱ（ＮＤⅡ）系弱毒疫苗点眼免疫，非免疫组雏鸡不免疫。定期收集免疫组和对非免疫组雏鸡肺脏和支气管，制做石蜡切片，苏木素伊红（ＨＥ）、阿尔森蓝－过碘酸雪夫氏（Ａｌｃｉｎｂｌｕｅ－ＰＡＳ）和酸性非特异酯酶（ＡＮＡＥ）染色。经组织学观察，免疫组雏鸡支气管结合淋巴组织（Ｂｒｏｎｃｈｕｓ－ａｓｓｏｃｉａｔｅｄｌｙｍｐｈｏｉｄｔｉｓｓｕｅ，ＢＡＬＴ）比非免疫组雏鸡的发生早、生长快、体积大、淋巴细胞数量多。ＢＡＬＴ中有ＡＮＡＥ阳性淋巴细胞。此结果证明抗原刺激能促进雏鸡ＢＡＬＴ的发育成熟，产生细胞免疫反应。Fify one-day-age male chickens were divided into two equal number groups, immunsed and controlled groups,chickens in the immunsed group was given Newcastle Disease vaccine (NDB1) by ocular route at one-day-age and the controlled was not. During 1 to 10-day-age samples of lungs and bronchile were collected from immunsed and controlled group at scheduled times to be stained with hematoxylin and eosin (HE) , Alcin blue-PAS, ANAE. Examined by gross and histology, bronchus-associated lymphoid tissue (BALT) appeared earlier, developed faster and had more lymphocytes in immunsed chickens than in the controlled. The results indicated that antigen stimulate could promote the development of BALT, cellular immune response could take place in BALT.

关 键 词：淋巴组织 雏鸡 新城疫疫苗 组织形态 

分 类 号：S831.1[农业科学—畜牧学]