地肤子皂苷抗过敏活性部位及量效关系的研究  

Study on Anti-allergic Active Fractions and Dose-effect Relationship of Fructus Kochia Scoparia

作　　者：刘建萍[1] 由宝昌[1] 黎星辉[2] 刘仲华[2] 王国辉[1] 张晓晖[1] 

机构地区：[1]中国农业大学烟台研究院,山东烟台264067 [2]茶学教育部重点实验室,湖南长沙410128

出　　处：《山东农业科学》2009年第8期49-52,共4页Shandong Agricultural Sciences

基　　金：国家"十一五"科技支撑计划(2006BAD06B01);教育部高校博士点基金(20060307024)项目资助

摘　　要：分别采用4-AP致小鼠过敏性皮肤瘙痒模型和组胺致小鼠足肿胀试验，探索地肤子总皂苷硅胶柱层析的活性部位和量效关系。结果表明，地肤子抗过敏活性成分主要集中在氯仿一甲醇为9：1的2、3BV，8：2的1—3BV，7：3的1—3BV洗脱液中；地肤子总皂苷抗过敏作用的有效阈剂量为32mg／kg，良效剂量为150mg／kg，且抗过敏性瘙痒作用比抗过敏介质的致炎作用强。The active fractions and the dose - effect relationship of Fructus Kochia Scoparia were studied by using the experimental model of the mice allergic cutaneous pruritus caused by 4 - AP and the test of mice foot swelling caused by histamine. The results showed that the anti -allergic active components mainly existed in the2 and 3 BV eluante of 9 ： 1 CH3Cl-MeOH, 1 -3 BV eluante of 8 ： 2 and 7 ： 3 CH3Cl- MeOH. The effective threshold dose of total saponin of Fructus Kochia Scoparia was 32 mg/kg and the moder- ate effective dose was 150 mg/kg. The results also showed that its resistance to the allergic pruritus was stronger than that to the infammafion - causing effect of anti - allergic medium.

关 键 词：地肤子 总皂苷 抗过敏作用 活性部位 量效关系 

分 类 号：R282.710.5[医药卫生—中药学]