新城疫病毒SGM01分离株F基因多肽重复区的序列分析和初步表达  

Sequence Analysis and Expression of Peptide Repeat Region of F Gene from Isolated Newcastle Disease Virus Strain SGM01

作　　者：袁小远[1] 欧阳文军[1] 张玉霞[1] 金维江[1] 秦卓明[1] 

机构地区：[1]山东省农业科学院家禽研究所,山东济南250023

出　　处：《山东农业科学》2009年第8期104-106,共3页Shandong Agricultural Sciences

基　　金：山东省农业科学院创新基金(2006YCX024);山东省农业科学院青年基金(2005YQ038)资助

摘　　要：从新城疫病毒(Newcastle disease virus,NDV)SGM01强毒分离株的F基因克隆出两段七肽重复序列(Heptad Repeat Region,HR)HR1和HR2,通过氨基酸序列分析显示:SGM01分离株的HR1区与F48E9、La-Sota的同源性分别为95.2%、93.7%;HR2区与F48E9、LaSota的同源性分别为89.1%、85.5%。将HR1和HR2分别插入融合表达载体pGEX-6p-1,在大肠杆菌BL21(DE3)中表达,得到预期大小的目的蛋白。这为研究HR1和HR2的结构以及其在F蛋白融合过程中所起的作用奠定了基础。Two heptad repeat fragments, HR1 and HR2, were amplified from F gene of isolated Newcastle disease virus （NDV） SGM01. The results of sequence analysis showed that the homology of HR1 with F48E9 and LaSota were 95.2% and 93.7% respectively, while those of HR2 were 89.1% and 85.5% respectively. The expected size protein was obtained by cloning HR1 and HR2 into the vector pGEX -6p - 1 and expressing in E. coli BL21 （DE3）. These results provided a theoretical basis for the study of structure and action of HR1 and HR2 during F protein fusion.

关 键 词：新城疫病毒 多肽重复区 序列分析 表达 

分 类 号：S436.421.19[农业科学—农业昆虫与害虫防治]