光老化成纤维细胞分泌的细胞外基质对HaCaT细胞增殖的影响  

Effects of ECM secreted by photoaging fibroblasts on the proliferation of HaCaT cells

作　　者：康健[1] 陈文琦[1] 夏济平[1] 李燕华[1] 杨波[1] 纪超[1] 宋秀祖 相文忠 万寅生[3] 毕志刚[1] 

机构地区：[1]南京医科大学第一附属医院皮肤科,南京210029 [2]Department of Biology, Providence College [3]杭州市第二医院皮肤科,310009

出　　处：《中国麻风皮肤病杂志》2009年第8期582-584,共3页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金资助项目(30671894);江苏省135医学重点学科项目经费资助

摘　　要：目的:评价紫外线诱导衰老成纤维细胞分泌的细胞外基质对HaCaT细胞增殖的促进作用。方法:连续UVB辐射诱导成纤维细胞发生应激性衰老(SIPS)。成纤维细胞接种后,制备细胞外基质(ECM)。将预处理的HaCaT细胞接种到包被ECM的平皿。流式细胞仪检测HaCaT细胞周期和凋亡,MTT法检测细胞的增殖。结果:UVB-SIPS成纤维细胞分泌的ECM对HaCaT细胞具有较强的促增殖作用,但对细胞凋亡无显著影响。结论:UVB-SIPS成纤维细胞分泌的ECM可以更强地促进HaCaT细胞的增殖。Objective： To determine the effect of extracellular matrices （ECM） secreted by UVB - stress induced premature senescent （UVB- SIPS） fibroblasts on the proliferation of HaCaT ceils. Methods： Stressaged fibroblasts （FBs） were induced by continuous exposure to UVB. After inoculation of fibroblasts, extracellular matrix （ECM） was prepared. Prepared HaCaT cells were shifted to plate dishes and were incubated. The apoptosis and cell - cycle of HaCaT cells were detected by flow cytometry method. The proliferation of HaCaT was measured with MTT assay. Results. ECM secreted by fibroblasts enhanced the proliferation of HaCaT cells, but had no significant effect on the apoptosis. HaCaT cultured in non - ECM, ECM from presenescent FBs and FBs in UVB - SIPS have undergone average 4.01%, 5.47% and 7.12% apoptosis, respectively. The HaCaT in UVB - SIPS group had the most significant increase of cell proportion in S phase （ P 〈 0.05）. Conclusion： ECM secreted by fibroblasts can promote the proliferation of HaCaT cells.

关 键 词：UVB 细胞外基质 成纤维细胞 HACAT细胞 衰老 凋亡 

分 类 号：R758.1[医药卫生—皮肤病学与性病学]