去卵巢大鼠海马CA1／CA2区ERK1／2的基础活性和诱导活性降低  

DECREASED BASIC ACTIVITY AND INDUCED ACTIVITY OF ERK1/2 PATHWAY IN HIPPOCAMPAL CA1/CA2 REGION OF OVARIECTOMIZED RATS

作　　者：张若楠[1] 宋士军[2] 李芳芳[2] 马俊骥[1] 李怡秋[1] 

机构地区：[1]河北医科大学基础医学院生物化学与分子生物学教研室,河北石家庄050017 [2]河北医科大学基础医学院生理教研室,河北石家庄050017

出　　处：《中国应用生理学杂志》2009年第3期411-415,共5页Chinese Journal of Applied Physiology

基　　金：河北省科学技术研究与发展指导计划(0527645)

摘　　要：目的:观察去卵巢大鼠空间学习记忆能力的变化与海马中胞外信号调节激酶1/2(ERK1/2)通路的关系。方法:雌性SD大鼠随机分为假手术组和去卵巢组,饲养4个月后采用Morris水迷宫测试空间学习记忆能力,于测试前将各组组内又分为训练组和非训练组,训练组用于测定经学习记忆训练诱发的ERK1/2的诱导活性,非训练组用于测定未经学习记忆训练时的ERK1/2的基础活性,Western blot方法检测海马CA1/CA2区p-ERK1/2蛋白及Raf激酶抑制蛋白(RKIP)的变化。结果:①与假手术组比较,去卵巢组大鼠的空间学习记忆能力明显下降(P<0.05)。②各组中的训练大鼠p-ERK1/2蛋白水平明显高于非训练大鼠(P<0.05)。③去卵巢组训练及非训练大鼠的p-ERK1/2蛋白水平均相应低于假手术组训练及非训练大鼠(P<0.05)。④去卵巢组RKIP蛋白表达水平明显高于假手术组(P<0.05)。结论:雌激素缺乏大鼠的空间学习记忆能力下降与海马CA1/CA2区ERK1/2通路的基础和诱导活性降低以及该通路的抑制蛋白RKIP的表达增加有关。Aim： To investigate the relationship between the spatial learning and memory and hippocampal ERK1/2 pathway activity in ovariectomized rats. Methods： Female SD rats were randomly divided into sham operated group （Sham group） and ovariectomized group （OVX group）, and fed 4 months. Then spatial learning and memory of rats were evaluated by the Morris water maze task. Rats in each group were randomly divided into training group and tmtraining group before the test. Induced activity of ERK 1/2 stimulated by learning and memory was detected in the training group, and basic activity of ERK 1/2 was detected in the untraining group. The protein expression of p-ERK 1/2 and Raf kinase inhibitor protein（RKIP） were assayed by Western blotting respectively. Results： ① During the training session the OVX rats held longer escape latenci than the sham rats did（ P 〈 0.05）. ② The relative level of p-ERK1/2 protein in training rats of the both groups was higher than that in untraining rats（ P 〈 0.05）. ③ The relative level of p-ERK1/2 protein both training and untraining rats in OVX group was lower than that in sham group correspondingly（ P 〈 0.05）. ④ Compared with sham group, the relative expression of RKIP in OVX group was significantly higher（ P 〈 0.05）. Conclusion： Spatial learning and memory deficits in ovariectomized rats might be correlated with the decreased basic and induced activity of ERK1/2 pathway and increased expression of RKIP in the CA1/CA2 region of hippocampus.

关 键 词：雌激素缺乏 海马 学习记忆 胞外信号调节激酶1/2 RAF激酶抑制蛋白 

分 类 号：R338.64[医药卫生—人体生理学] Q257[医药卫生—基础医学]