多次低剂量照射对雄性糖尿病大鼠脾细胞凋亡和免疫因子的影响  

Effects of multiple low dose radiation on the apoptosis of splenocytes and immune factor in male diabetic rats

作　　者：李艳博[1] 郭彩霞[2] 董丽华[3] 王剑锋[1] 刘淑春[1] 吕喆[1] 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,长春130021 [2]吉林大学公共卫生学院卫生毒理学教研室,长春130021 [3]吉林大学第一医院肿瘤中心放疗科

出　　处：《中华放射医学与防护杂志》2009年第4期364-367,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：基金项目：国家自然科学基金（30570546）

摘　　要：目的观察多次低剂量辐射（LDR）对糖尿病（DM）大鼠脾细胞凋亡和免疫因子的影响。方法大鼠随机分为对照组、DM组和DM＋LDR组；剂量分别为25、50和75mGy，共照射15次；照射后4周，采用流式细胞术检测脾细胞凋亡和TCRaB百分数的变化，酶联免疫吸附法（ELISA）检测血清和脾细胞培养上清IL-2含量的变化。结果与对照组相比，DM和DM＋LDR两组大鼠体重均下降，尤以DM组明显。DM＋LDR组大鼠血糖水平虽明显高于对照组（t25=23．321、t50=18．329、t75=9．23，P〈0．01），但显著低于DM组（t25=3．574、t50=4．593、t75=5．577，P〈0．01）。同时发现：与对照组相比，DM＋LDR各组脾细胞凋亡增加，其中DM＋50mGy组增加明显（t50=4．102，P〈O．01）。血清IL-2含量也增加，但是差异均无统计学意义。脾细胞培养上清IL-2含量明显下降（t25=7．778、t50=7．411、t，5=8．325，P〈0．01）。与DM组相比，DM＋LDR各组脾细胞凋亡和TCRαβ百分数均明显降低（凋亡：t25：4．772、t50=3．346、t75=6．778；TCRαβ：t25=3．381、t50=5．807、t75=2．356，P〈0．05-P〈0．01）。血清IL-2含量呈下降趋势；脾细胞培养上清IL-2含量均有升高趋势。结论多次LDR能够削弱糖尿病造成的大鼠体重减轻和血糖升高，降低糖尿病所致的脾细胞凋亡，并能调节脾脏免疫因子，改善其失衡状态。Objective To explore the effect of muhiple low dose radiation （LDR） on the apeptosis of splenocytes and immune factors in diabetes meUitus （DM） rats. Methods The rats were randomly divided into control, DM and DM ＋ LDR groups. The irradiation doses were 25, 50 and 75 mGy, and the irradiated times were 15. At the fourth weekend after the DM rats irradiated, the apoptotie rate and TCRαβ percentage of splenocytes were detected by flow cytometry, and the content of IL-2 in both serum and supernatant of cultured splenocytes were detected by ELISA. Results Compared with that in the control, the body weight （BW） decreased in the DM and DM ＋ LDR groups, particularly in DM group. The blood glucose （BG） level in the DM ＋ LDR groups was higher than that in the control, but decreased significantly as compared with that in the DM group （P 〈 0.01 ）. As compared with those in the control, the apoptotic rate in DM ＋ 50 mGy （P 〈 0.05） and the content of serum IL-2 in DM ＋ 75 mGy group （P 〈 0.01 ） all increased significantly, while the content of IL-2 in superuatant of cultured splenocytes decreased significantly in the DM ＋ LDR groups. Compared with those in the DM group, the apoptotic rate and the percentage of TCRαβ in splenocytes in the DM ＋ LDR groups （ P 〈 0.01-P 〈 0.001） and the content of IL-2 in serum in DM ＋ 50 mGy group （P 〈 0.01） decreased significantly. Conchmions The multiple LDR could weaken the loss of BW and increase of BG caused by DM, decrease the splenocyte apeptosis induced by DM, and regulate the immune factors.

关 键 词：低剂量辐射 糖尿病 脾脏 凋亡 免疫因子 

分 类 号：R587.1[医药卫生—内分泌] R995[医药卫生—内科学]