辐射损伤后小鼠T细胞功能亚群及其IL-4和IFN-γ的变化  

Changes of T-lymphocyte function subsets and IL-4 and IFN-γ after irradiation injury

作　　者：郑辉[1] 甄荣[1] 涂序珉[1] 张志兴[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《中华放射医学与防护杂志》2009年第4期386-388,共3页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的观察不同剂量辐射损伤后小鼠T细胞功能亚群、细胞因子IL-4和IFN-γ的变化。方法C57BL／6j小鼠分为假照射组和辐射损伤模型组。采用60Coγ线照射诱导辐射损伤模型。吸收剂量分别为0．7、1．4、2，8及5．6Gy。应用细胞表面标志和细胞内因子标记结合流式细胞仪分析急性照射损伤和损伤恢复期小鼠脾CD3＋、CD4＋、CD8＋T细胞功能亚群，以及细胞因子IL4和IFN-γ的变化。结果①辐射损伤后CD3＋、CD4＋及CD8＋有明显的减低，其改变幅度与受照剂量有关。②照射后1d，IFN-γ降低幅度明显高于IL-4，受照剂量大于2．8Gy组，IL-4／IFN-γ比值较空白对照组明显升高。③辐射对淋巴细胞功能亚群、细胞因子IL-4和IFN-γ的损伤在照射后25d有不同程度的恢复，但与假照射组比较仍有明显差别。结论电离辐射可使淋巴细胞亚群CD3＋，CD4＋，CD8＋、CD4＋／CD8＋、细胞因子IL-4和IFN-γ发生改变，并诱发受照小鼠免疫功能低下，特别是使细胞因子IL-4和IFN-γ发生失衡，再次证实辐射损伤后小鼠脾Th1／Th2模式向Th2免疫反应漂移的现象。Objective To observe the effects of T-lymphocyte function subsets, IL-4 and IFN-γ cell factor in different dose and stage after irradiation. Methods The C57BL/6j mice were divided into shammed irradiation group and model groups. The radiation hurt model was induced by 60Co gamma rays （0.7, 1.4, 2.8 and 5.6 Gy）. The changes of T- lymphocyte subsets CD3, CD4, CD8, IL-4 and IFN-γ in spleen cells were analyzed by flow cytometry in acute injury stage and recovery stage after irradiation. Results The lymphocyte subsets CD3 ＋ , CD4 ＋ and CD8 ＋ decreased after irradiation, which were related to the irradiation dose. At 1 day after irradiation, the decreasing level of IFN-γwas higher than that of IL-4. When irradiation dose was over 2.8 Gy, IL-4 / IFN-γ showed a markedly increased compared with control group. At 25 days after irradiation, CD3 ＋ ,CD4＋ ,CD8 ＋ ,CD 4＋/CD8 ＋ , IL-4 and IFN-γ recovered obviously, but they did not recover to the normal level of shammed irradiation group, Conclusions The depression of mouse immune function induced by γ-irradiation might be caused by changes of CD3, CD4, CD8 and CD4/CD8 ratio, especially the imbalance of IL- 4 and IFN-γ.

关 键 词：辐射 免疫 损伤 T淋巴细胞亚群 白细胞介素-4(IL-4) IFN-Γ 

分 类 号：R285.5[医药卫生—中药学]